Detailed information of TA system TA233018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2302482..2303515
• Replicon: chromosome
• Accession: NZ_CP091237
• Organism: Enterococcus faecalis strain 1521

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: LQ062_RS10940
• Protein ID: WP_142429825.1
• Coordinates: 2302862..2303515 (+)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: LQ062_RS10935
• Protein ID: WP_010824178.1
• Coordinates: 2302482..2302865 (+)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ062_RS10905 (2298831)	2298831..2299211	-	381	WP_002402315.1	DUF86 domain-containing protein	-
LQ062_RS10910 (2299201)	2299201..2299527	-	327	WP_002394268.1	nucleotidyltransferase domain-containing protein	-
LQ062_RS10915 (2299587)	2299587..2299880	-	294	WP_002394269.1	hypothetical protein	-
LQ062_RS10920 (2300659)	2300659..2301075	-	417	WP_219259950.1	DUF961 family protein	-
LQ062_RS10925 (2301075)	2301075..2301401	-	327	WP_219259948.1	hypothetical protein	-
LQ062_RS10930 (2301487)	2301487..2301738	-	252	WP_219259946.1	hypothetical protein	-
LQ062_RS10935 (2302482)	2302482..2302865	+	384	WP_010824178.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ062_RS10940 (2302862)	2302862..2303515	+	654	WP_142429825.1	ImmA/IrrE family metallo-endopeptidase	Toxin
LQ062_RS10945 (2303554)	2303554..2303889	+	336	WP_002402309.1	helix-turn-helix domain-containing protein	-
LQ062_RS10950 (2303927)	2303927..2305258	-	1332	WP_219259944.1	FAD-dependent oxidoreductase	-
LQ062_RS10955 (2305362)	2305362..2305598	-	237	Protein_2131	aldo/keto reductase	-
LQ062_RS10960 (2305670)	2305670..2306719	-	1050	WP_219259942.1	hypothetical protein	-
LQ062_RS10965 (2306961)	2306961..2307188	-	228	WP_219259940.1	hypothetical protein	-
LQ062_RS10970 (2307280)	2307280..2307474	-	195	Protein_2134	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (80-181)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 26078.05 Da
• Isoelectric Point: 6.4968

>T233018 WP_142429825.1 NZ_CP091237:2302862-2303515 [Enterococcus faecalis]
MIGLLLVNFISKELYFEAFNFSNELIKEVSYYSSKNIEQVKCFDIEKYVKEVEDVEFVEYTFQKQLKRRMLGSISKVDDE
VIITTNKELMLERKNFTKMHEIMHYYIDIPKINNATHTFSDILLKNGYLMEDFPKEYRANVGASMLMANDQALFYALKKF
YSFEEIAQYFFMSKSALRNRLIEHLMYVNNCTFAHANTLFNNYYFHKETDIYRFIFN

Nucleotide

• Length: 654 bp

>T233018 NZ_CP128518:1571879-1572055 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 15032.08 Da
• Isoelectric Point: 5.3750

>AT233018 WP_010824178.1 NZ_CP091237:2302482-2302865 [Enterococcus faecalis]
VQPLAKRLQLLREEKEWTKTYVAKQLGLNNLGTYANWEYGTREPDSEMLSKIASLYDVSTDFLLGRTEERIHLNDKEKIG
KNIISHFRLNTSDMDIEDIEELEEELIDFQDFLIKKAKEKKKRNKKD

Nucleotide

• Length: 384 bp

>AT233018 NZ_CP128518:1572111-1572527 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA