Detailed information of TA system TA233006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2822475..2823046
• Replicon: chromosome
• Accession: NZ_CP091235
• Organism: Enterococcus faecalis strain 705

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LQ054_RS13550
• Protein ID: WP_002354774.1
• Coordinates: 2822475..2822816 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: LQ054_RS13555
• Protein ID: WP_002354773.1
• Coordinates: 2822816..2823046 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ054_RS13545 (2818490)	2818490..2822104	-	3615	WP_249455875.1	DNA-directed RNA polymerase subunit beta	-
LQ054_RS13550 (2822475)	2822475..2822816	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LQ054_RS13555 (2822816)	2822816..2823046	-	231	WP_002354773.1	hypothetical protein	Antitoxin
LQ054_RS13560 (2823220)	2823220..2823435	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LQ054_RS13565 (2823574)	2823574..2824566	+	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LQ054_RS13570 (2824733)	2824733..2825371	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
LQ054_RS13575 (2826056)	2826056..2827672	+	1617	WP_002378864.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T233006 WP_002354774.1 NZ_CP091235:c2822816-2822475 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T233006 NZ_CP128516:564139-564435 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT233006 WP_002354773.1 NZ_CP091235:c2823046-2822816 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT233006 NZ_CP128516:564419-564661 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812