Detailed information of TA system TA233001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 1569180..1570288
• Replicon: chromosome
• Accession: NZ_CP091235
• Organism: Enterococcus faecalis strain 705

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A193AUR0
• Locus tag: LQ054_RS07585
• Protein ID: WP_002378351.1
• Coordinates: 1569180..1570049 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LQ054_RS07590
• Protein ID: WP_000205227.1
• Coordinates: 1570064..1570288 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ054_RS07565 (1565451)	1565451..1566770	-	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-
LQ054_RS07570 (1566998)	1566998..1567399	-	402	WP_002401582.1	phosphoribosyltransferase family protein	-
LQ054_RS07575 (1567569)	1567569..1568432	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LQ054_RS07580 (1568465)	1568465..1569199	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LQ054_RS07585 (1569180)	1569180..1570049	-	870	WP_002378351.1	nucleotidyltransferase domain-containing protein	Toxin
LQ054_RS07590 (1570064)	1570064..1570288	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ054_RS07595 (1570431)	1570431..1570517	-	87	Protein_1468	site-specific recombinase	-
LQ054_RS07600 (1570706)	1570706..1572007	+	1302	Protein_1469	ISL3 family transposase	-
LQ054_RS07605 (1572316)	1572316..1573119	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LQ054_RS07610 (1573173)	1573173..1574657	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	erm(B) / ant(6)-Ia / lnu(B) / lsa(E) / aph(3')-III / aac(6')-aph(2'') / cat	-	1558509..1590725	32216
	-	inside	Genomic island	ant(6)-Ia / lnu(B) / lsa(E) / aph(3')-III / aac(6')-aph(2'') / erm(B) / cat	-	1567400..1589783	22383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32912.50 Da
• Isoelectric Point: 4.7903

>T233001 WP_002378351.1 NZ_CP091235:c1570049-1569180 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T233001 NZ_CP128515:1568146-1568328 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT233001 WP_000205227.1 NZ_CP091235:c1570288-1570064 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT233001 NZ_CP128515:1568367-1568786 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A193AUR0