Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 300249..300832 Replicon chromosome
Accession NC_011740
Organism Escherichia fergusonii ATCC 35469

Toxin (Protein)


Gene name mazF Uniprot ID B7LWP2
Locus tag EFER_RS01635 Protein ID WP_000254732.1
Coordinates 300497..300832 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag EFER_RS01630 Protein ID WP_000581937.1
Coordinates 300249..300497 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFER_RS01620 296588..297886 + 1299 WP_000046872.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
EFER_RS01625 297937..300171 + 2235 WP_000226845.1 GTP diphosphokinase -
EFER_RS01630 300249..300497 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
EFER_RS01635 300497..300832 + 336 WP_000254732.1 endoribonuclease MazF Toxin
EFER_RS01640 300903..301694 + 792 WP_001071671.1 nucleoside triphosphate pyrophosphohydrolase -
EFER_RS01645 301922..303559 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
EFER_RS01650 303647..304945 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 305000..306328 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12156.08 Da        Isoelectric Point: 7.7128

>T23299 WP_000254732.1 NC_011740:300497-300832 [Escherichia fergusonii ATCC 35469]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSDQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T23299 NC_011740:300497-300832 [Escherichia fergusonii ATCC 35469]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGATCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTGAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT23299 WP_000581937.1 NC_011740:300249-300497 [Escherichia fergusonii ATCC 35469]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT23299 NC_011740:300249-300497 [Escherichia fergusonii ATCC 35469]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B7LWP2

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References