Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2853863..2854434 Replicon chromosome
Accession NZ_CP091231
Organism Enterococcus faecalis strain 732

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag LQ055_RS14105 Protein ID WP_002360937.1
Coordinates 2853863..2854204 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S4CGQ1
Locus tag LQ055_RS14110 Protein ID WP_002367500.1
Coordinates 2854204..2854434 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ055_RS14100 (2849878) 2849878..2853492 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
LQ055_RS14105 (2853863) 2853863..2854204 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LQ055_RS14110 (2854204) 2854204..2854434 - 231 WP_002367500.1 hypothetical protein Antitoxin
LQ055_RS14115 (2854489) 2854489..2854668 - 180 WP_111997057.1 hypothetical protein -
LQ055_RS14120 (2854848) 2854848..2855063 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
LQ055_RS14125 (2855202) 2855202..2856194 + 993 WP_002389493.1 biotin--[acetyl-CoA-carboxylase] ligase -
LQ055_RS14130 (2856458) 2856458..2857096 - 639 WP_002378863.1 lytic polysaccharide monooxygenase -
LQ055_RS14135 (2857782) 2857782..2859398 + 1617 WP_002389442.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T232978 WP_002360937.1 NZ_CP091231:c2854204-2853863 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T232978 NZ_CP128508:2401149-2401442 [Pseudomonas asiatica]
GTGCCACGACTGATTGTCACTGAAGGCGCGGCGAAGGGCTTGGAGCGTTGCCGGCGATTCCTCTCCGATAAAGATCCGCA
GGTCGCGCGCCGCGCTGCACAGGCAATCGAGCGCCAATTCGTTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCATTTC
CAGATCTGCCAGAGCTGCGTGAGCTCATCATCGAGTTTGGTGACTCAGGTTACGTCGCGCTGTACCGCTATGAGCGCGCA
GATGACACCACCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8704.69 Da        Isoelectric Point: 4.3313

>AT232978 WP_002367500.1 NZ_CP091231:c2854434-2854204 [Enterococcus faecalis]
MLTTKSRRQGNSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT232978 NZ_CP128508:2400889-2401161 [Pseudomonas asiatica]
ATGGCCACTTCCATCAAAATTGACGACGAGCTGAAAGGTCGCATTCAGCATTTGGCTGGTTTGCGGCAGCGTTCATCTCA
CTGGATCATGCGTGAGGCGATCACCCAGTACGTCGAGCGTGAGGAGGCCCGCGAGAGCTTCAAACAAGAAGCGCTGGCAT
CTTGGGCTGCGTATCAGGAAACCGGCAGGCATTTGACCGGCCAAGAGGCTCGCACCTGGCTTGGTAGCTGGGGTACAGAT
GCTGAGGCAGAGCTTCCCAAGTGCCACGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB S4CGQ1

References