Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 449781..450363 Replicon chromosome
Accession NZ_CP091231
Organism Enterococcus faecalis strain 732

Toxin (Protein)


Gene name zeta Uniprot ID A0A0M2AE74
Locus tag LQ055_RS02350 Protein ID WP_002355414.1
Coordinates 450055..450363 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag LQ055_RS02345 Protein ID WP_002326825.1
Coordinates 449781..450053 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ055_RS02320 (445062) 445062..445790 - 729 WP_002355404.1 GntR family transcriptional regulator -
LQ055_RS02325 (445967) 445967..446893 + 927 WP_002355406.1 hypothetical protein -
LQ055_RS02330 (446910) 446910..448193 + 1284 WP_002363033.1 PTS sugar transporter subunit IIC -
LQ055_RS02335 (448582) 448582..449478 + 897 WP_002363034.1 ParA family protein -
LQ055_RS02340 (449555) 449555..449764 + 210 WP_002363035.1 peptide-binding protein -
LQ055_RS02345 (449781) 449781..450053 + 273 WP_002326825.1 antitoxin Antitoxin
LQ055_RS02350 (450055) 450055..450363 + 309 WP_002355414.1 zeta toxin family protein Toxin
LQ055_RS02355 (450443) 450443..450850 - 408 WP_224571294.1 tyrosine-type recombinase/integrase -
LQ055_RS02360 (450917) 450917..451417 - 501 WP_002363039.1 HAD family hydrolase -
LQ055_RS02365 (451422) 451422..452189 - 768 WP_002355416.1 DeoR/GlpR family DNA-binding transcription regulator -
LQ055_RS02370 (452676) 452676..453101 + 426 WP_002355418.1 galactose-6-phosphate isomerase subunit LacA -
LQ055_RS02375 (453118) 453118..453633 + 516 WP_002345825.1 galactose-6-phosphate isomerase subunit LacB -
LQ055_RS02380 (453644) 453644..454576 + 933 WP_002363040.1 tagatose-6-phosphate kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element ClpL bsh 417594..496523 78929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-99)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11525.91 Da        Isoelectric Point: 5.7251

>T232958 WP_002355414.1 NZ_CP091231:450055-450363 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKHATSYSNQLVKLN

Download         Length: 309 bp

>T232958 NZ_CP128503:c3762840-3762490 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT232958 WP_002326825.1 NZ_CP091231:449781-450053 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT232958 NZ_CP128503:c3763127-3762846 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AE74

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References