Detailed information of TA system TA232958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 449781..450363
• Replicon: chromosome
• Accession: NZ_CP091231
• Organism: Enterococcus faecalis strain 732

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: A0A0M2AE74
• Locus tag: LQ055_RS02350
• Protein ID: WP_002355414.1
• Coordinates: 450055..450363 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL19
• Locus tag: LQ055_RS02345
• Protein ID: WP_002326825.1
• Coordinates: 449781..450053 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ055_RS02320 (445062)	445062..445790	-	729	WP_002355404.1	GntR family transcriptional regulator	-
LQ055_RS02325 (445967)	445967..446893	+	927	WP_002355406.1	hypothetical protein	-
LQ055_RS02330 (446910)	446910..448193	+	1284	WP_002363033.1	PTS sugar transporter subunit IIC	-
LQ055_RS02335 (448582)	448582..449478	+	897	WP_002363034.1	ParA family protein	-
LQ055_RS02340 (449555)	449555..449764	+	210	WP_002363035.1	peptide-binding protein	-
LQ055_RS02345 (449781)	449781..450053	+	273	WP_002326825.1	antitoxin	Antitoxin
LQ055_RS02350 (450055)	450055..450363	+	309	WP_002355414.1	zeta toxin family protein	Toxin
LQ055_RS02355 (450443)	450443..450850	-	408	WP_224571294.1	tyrosine-type recombinase/integrase	-
LQ055_RS02360 (450917)	450917..451417	-	501	WP_002363039.1	HAD family hydrolase	-
LQ055_RS02365 (451422)	451422..452189	-	768	WP_002355416.1	DeoR/GlpR family DNA-binding transcription regulator	-
LQ055_RS02370 (452676)	452676..453101	+	426	WP_002355418.1	galactose-6-phosphate isomerase subunit LacA	-
LQ055_RS02375 (453118)	453118..453633	+	516	WP_002345825.1	galactose-6-phosphate isomerase subunit LacB	-
LQ055_RS02380 (453644)	453644..454576	+	933	WP_002363040.1	tagatose-6-phosphate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ClpL	bsh	417594..496523	78929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-99)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11525.91 Da
• Isoelectric Point: 5.7251

>T232958 WP_002355414.1 NZ_CP091231:450055-450363 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKHATSYSNQLVKLN

Nucleotide

• Length: 309 bp

>T232958 NZ_CP128503:c3762840-3762490 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10720.17 Da
• Isoelectric Point: 4.4865

>AT232958 WP_002326825.1 NZ_CP091231:449781-450053 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Nucleotide

• Length: 273 bp

>AT232958 NZ_CP128503:c3763127-3762846 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A0M2AE74

Antitoxin

• AlphaFold DB: A0A0M2AF93