232955

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 95956..97093 Replicon plasmid p661-a
Accession NZ_CP091228
Organism Enterococcus faecalis strain 661

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag LQ066_RS13855 Protein ID WP_002332783.1
Coordinates 95956..96819 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag LQ066_RS13860 Protein ID WP_002326825.1
Coordinates 96821..97093 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ066_RS13815 (LQ066_13815) 91325..91441 - 117 Protein_105 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
LQ066_RS13820 (LQ066_13820) 91611..92348 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
LQ066_RS13825 (LQ066_13825) 92473..92556 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
LQ066_RS13830 (LQ066_13830) 92679..92846 - 168 Protein_108 peptide-binding protein -
LQ066_RS13835 (LQ066_13835) 92980..93606 - 627 Protein_109 DNA topoisomerase -
LQ066_RS13840 (LQ066_13840) 93682..94362 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
LQ066_RS13845 (LQ066_13845) 94396..94902 - 507 WP_002415429.1 trimethoprim-resistant dihydrofolate reductase DfrG -
LQ066_RS13850 (LQ066_13850) 95199..95516 - 318 WP_002326830.1 hypothetical protein -
LQ066_RS13855 (LQ066_13855) 95956..96819 - 864 WP_002332783.1 zeta toxin family protein Toxin
LQ066_RS13860 (LQ066_13860) 96821..97093 - 273 WP_002326825.1 antitoxin Antitoxin
LQ066_RS13865 (LQ066_13865) 97111..97326 - 216 WP_001835296.1 peptide-binding protein -
LQ066_RS13870 (LQ066_13870) 97418..98314 - 897 WP_002326827.1 ParA family protein -
LQ066_RS13875 (LQ066_13875) 98417..98677 - 261 Protein_117 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
LQ066_RS13880 (LQ066_13880) 98847..99584 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
LQ066_RS13885 (LQ066_13885) 99709..99792 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
LQ066_RS14115 99841..99924 - 84 Protein_120 MLS leader peptide -
LQ066_RS13890 (LQ066_13890) 100224..100595 - 372 WP_002358205.1 hypothetical protein -
LQ066_RS13895 (LQ066_13895) 100588..101433 - 846 WP_000239313.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cat / tet(L) / tet(M) / erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') / lnu(B) / lsa(E) / dfrG - 1..101904 101904
- inside IS/Tn erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') / lnu(B) / lsa(E) / dfrG - 74797..99584 24787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T232955 WP_002332783.1 NZ_CP091228:c96819-95956 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T232955 NZ_CP128501:c1199519-1198773 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTATCAAATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTATGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGGCAATACTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGAGGGAAATATTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGCCTTAA
CCGGAAAGACGTCTACTATGATCAAAAAGGAAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCAGTATGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGATAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT232955 WP_002326825.1 NZ_CP091228:c97093-96821 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT232955 NZ_CP128501:c1198773-1198603 [Bacillus amyloliquefaciens]
ATGGAGCAGGCGTTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCACACCGCCAGAATTTTCAAAGAAAATGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References