Detailed information of TA system TA232954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 81386..82494
• Replicon: plasmid p661-a
• Accession: NZ_CP091228
• Organism: Enterococcus faecalis strain 661

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: LQ066_RS13755
• Protein ID: WP_000233000.1
• Coordinates: 81386..82255 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LQ066_RS13760
• Protein ID: WP_000205227.1
• Coordinates: 82270..82494 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ066_RS13725 (LQ066_13725)	77171..77713	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
LQ066_RS13730 (LQ066_13730)	77710..78507	-	798	Protein_88	aminoglycoside 6-adenylyltransferase	-
LQ066_RS13735 (LQ066_13735)	78677..80116	-	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
LQ066_RS13740 (LQ066_13740)	80117..80509	-	393	WP_000393259.1	GNAT family N-acetyltransferase	-
LQ066_RS13745 (LQ066_13745)	80528..80638	-	111	Protein_91	aminoglycoside 6-adenylyltransferase	-
LQ066_RS13750 (LQ066_13750)	80671..81405	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LQ066_RS13755 (LQ066_13755)	81386..82255	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
LQ066_RS13760 (LQ066_13760)	82270..82494	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ066_RS13765 (LQ066_13765)	82637..82866	-	230	Protein_95	hypothetical protein	-
LQ066_RS13770 (LQ066_13770)	82932..84214	+	1283	Protein_96	ISL3 family transposase	-
LQ066_RS13775 (LQ066_13775)	84523..85326	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LQ066_RS13780 (LQ066_13780)	85380..86864	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	cat / tet(L) / tet(M) / erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') / lnu(B) / lsa(E) / dfrG	-	1..101904	101904
	-	inside	IS/Tn	erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') / lnu(B) / lsa(E) / dfrG	-	74797..99584	24787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T232954 WP_000233000.1 NZ_CP091228:c82255-81386 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T232954 NZ_CP128501:338193-338543 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT232954 WP_000205227.1 NZ_CP091228:c82494-82270 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT232954 NZ_CP128501:337906-338187 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: R3JIN1