Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | Xre-MNT/HTH_26(antitoxin) |
| Location | 81386..82494 | Replicon | plasmid p661-a |
| Accession | NZ_CP091228 | ||
| Organism | Enterococcus faecalis strain 661 | ||
Toxin (Protein)
| Gene name | MNTss | Uniprot ID | R3JIN1 |
| Locus tag | LQ066_RS13755 | Protein ID | WP_000233000.1 |
| Coordinates | 81386..82255 (-) | Length | 290 a.a. |
Antitoxin (Protein)
| Gene name | Xress | Uniprot ID | - |
| Locus tag | LQ066_RS13760 | Protein ID | WP_000205227.1 |
| Coordinates | 82270..82494 (-) | Length | 75 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LQ066_RS13725 (LQ066_13725) | 77171..77713 | - | 543 | WP_000627290.1 | streptothricin N-acetyltransferase Sat4 | - |
| LQ066_RS13730 (LQ066_13730) | 77710..78507 | - | 798 | Protein_88 | aminoglycoside 6-adenylyltransferase | - |
| LQ066_RS13735 (LQ066_13735) | 78677..80116 | - | 1440 | WP_001028144.1 | aminoglycoside O-phosphotransferase APH(2'')-Ia | - |
| LQ066_RS13740 (LQ066_13740) | 80117..80509 | - | 393 | WP_000393259.1 | GNAT family N-acetyltransferase | - |
| LQ066_RS13745 (LQ066_13745) | 80528..80638 | - | 111 | Protein_91 | aminoglycoside 6-adenylyltransferase | - |
| LQ066_RS13750 (LQ066_13750) | 80671..81405 | - | 735 | WP_000662263.1 | class I SAM-dependent methyltransferase | - |
| LQ066_RS13755 (LQ066_13755) | 81386..82255 | - | 870 | WP_000233000.1 | nucleotidyltransferase domain-containing protein | Toxin |
| LQ066_RS13760 (LQ066_13760) | 82270..82494 | - | 225 | WP_000205227.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| LQ066_RS13765 (LQ066_13765) | 82637..82866 | - | 230 | Protein_95 | hypothetical protein | - |
| LQ066_RS13770 (LQ066_13770) | 82932..84214 | + | 1283 | Protein_96 | ISL3 family transposase | - |
| LQ066_RS13775 (LQ066_13775) | 84523..85326 | - | 804 | WP_002294514.1 | lincosamide nucleotidyltransferase Lnu(B) | - |
| LQ066_RS13780 (LQ066_13780) | 85380..86864 | - | 1485 | WP_002294513.1 | ABC-F type ribosomal protection protein Lsa(E) | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | cat / tet(L) / tet(M) / erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') / lnu(B) / lsa(E) / dfrG | - | 1..101904 | 101904 | |
| - | inside | IS/Tn | erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') / lnu(B) / lsa(E) / dfrG | - | 74797..99584 | 24787 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 290 a.a. Molecular weight: 32926.57 Da Isoelectric Point: 4.8781
>T232954 WP_000233000.1 NZ_CP091228:c82255-81386 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
Download Length: 870 bp
>T232954 NZ_CP128501:338193-338543 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG
Antitoxin
Download Length: 75 a.a. Molecular weight: 8365.68 Da Isoelectric Point: 4.5510
>AT232954 WP_000205227.1 NZ_CP091228:c82494-82270 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE
Download Length: 225 bp
>AT232954 NZ_CP128501:337906-338187 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|