Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 68190..68761 Replicon plasmid p661-a
Accession NZ_CP091228
Organism Enterococcus faecalis strain 661

Toxin (Protein)


Gene name mazF Uniprot ID A0A2Z2CGM4
Locus tag LQ066_RS13640 Protein ID WP_002362432.1
Coordinates 68190..68531 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag LQ066_RS13645 Protein ID WP_002362431.1
Coordinates 68531..68761 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ066_RS13615 (LQ066_13615) 63204..63899 - 696 WP_002405176.1 Fic family protein -
LQ066_RS13620 (LQ066_13620) 63948..64547 - 600 WP_002405175.1 tyrosine-type recombinase/integrase -
LQ066_RS13630 (LQ066_13630) 66273..66875 - 603 WP_002362434.1 Fic family protein -
LQ066_RS13635 (LQ066_13635) 67140..68078 - 939 WP_002362433.1 hypothetical protein -
LQ066_RS13640 (LQ066_13640) 68190..68531 - 342 WP_002362432.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LQ066_RS13645 (LQ066_13645) 68531..68761 - 231 WP_002362431.1 hypothetical protein Antitoxin
LQ066_RS13650 (LQ066_13650) 68965..69585 + 621 WP_002367784.1 recombinase family protein -
LQ066_RS13655 (LQ066_13655) 69575..69889 + 315 WP_002367785.1 hypothetical protein -
LQ066_RS13660 (LQ066_13660) 69883..70089 + 207 WP_002367786.1 hypothetical protein -
LQ066_RS13665 (LQ066_13665) 70249..70443 + 195 WP_002367787.1 hypothetical protein -
LQ066_RS13670 (LQ066_13670) 70455..70646 + 192 WP_002367788.1 hypothetical protein -
LQ066_RS13675 (LQ066_13675) 70816..71031 + 216 WP_002367791.1 leucocin A/sakacin P family class II bacteriocin -
LQ066_RS13680 (LQ066_13680) 71032..71373 + 342 WP_002367792.1 bacteriocin immunity protein -
LQ066_RS13685 (LQ066_13685) 71789..72307 + 519 WP_002367793.1 hypothetical protein -
LQ066_RS13690 (LQ066_13690) 72255..72470 + 216 WP_002415356.1 hypothetical protein -
LQ066_RS13695 (LQ066_13695) 72562..72648 + 87 WP_012881081.1 type I toxin-antitoxin system Fst family toxin -
LQ066_RS13700 (LQ066_13700) 72905..73201 + 297 WP_002367795.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cat / tet(L) / tet(M) / erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') / lnu(B) / lsa(E) / dfrG - 1..101904 101904


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13244.44 Da        Isoelectric Point: 8.0113

>T232953 WP_002362432.1 NZ_CP091228:c68531-68190 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T232953 NZ_CP128500:c3358549-3358199 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT232953 WP_002362431.1 NZ_CP091228:c68761-68531 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp

>AT232953 NZ_CP128500:c3358836-3358555 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z2CGM4

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References