Detailed information of TA system TA232942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2664863..2665434
• Replicon: chromosome
• Accession: NZ_CP091227
• Organism: Enterococcus faecalis strain 661

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LQ066_RS12825
• Protein ID: WP_002354774.1
• Coordinates: 2664863..2665204 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: LQ066_RS12830
• Protein ID: WP_002354773.1
• Coordinates: 2665204..2665434 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ066_RS12820 (2660878)	2660878..2664492	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
LQ066_RS12825 (2664863)	2664863..2665204	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LQ066_RS12830 (2665204)	2665204..2665434	-	231	WP_002354773.1	hypothetical protein	Antitoxin
LQ066_RS12835 (2665760)	2665760..2665975	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LQ066_RS12840 (2666114)	2666114..2667106	+	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LQ066_RS12845 (2667173)	2667173..2667805	-	633	WP_002358972.1	RloB family protein	-
LQ066_RS12850 (2667814)	2667814..2669109	-	1296	WP_002410675.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T232942 WP_002354774.1 NZ_CP091227:c2665204-2664863 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T232942 NZ_CP128487:c5209170-5208787 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT232942 WP_002354773.1 NZ_CP091227:c2665434-2665204 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT232942 NZ_CP128487:c5209412-5209170 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812