Detailed information of TA system TA023294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 1305929..1306506
• Replicon: chromosome
• Accession: NC_011729
• Organism: Cyanothece sp. PCC 7424

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B7K775
• Locus tag: PCC7424_RS05835
• Protein ID: WP_012598589.1
• Coordinates: 1305929..1306189 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PCC7424_RS05840
• Protein ID: WP_012598590.1
• Coordinates: 1306183..1306506 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCC7424_RS05815	1301961..1302977	-	1017	WP_012598585.1	chemotaxis protein CheB	-
PCC7424_RS05820	1303410..1304333	+	924	WP_012598586.1	NAD(P)H-dependent glycerol-3-phosphate dehydrogenase	-
PCC7424_RS05825	1304384..1305322	+	939	WP_203457610.1	hypothetical protein	-
PCC7424_RS31330	1305616..1305813	+	198	WP_203457590.1	hypothetical protein	-
PCC7424_RS05835	1305929..1306189	+	261	WP_012598589.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
PCC7424_RS05840	1306183..1306506	+	324	WP_012598590.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
PCC7424_RS05845	1306626..1308017	+	1392	WP_012598591.1	malate dehydrogenase	-
PCC7424_RS05850	1308082..1308813	+	732	WP_041237657.1	D-alanyl-D-alanine carboxypeptidase family protein	-
PCC7424_RS05855	1308873..1309730	+	858	WP_012598593.1	SWIM zinc finger family protein	-
PCC7424_RS05860	1309902..1310315	-	414	WP_012598594.1	DUF202 domain-containing protein	-
PCC7424_RS05865	1310327..1310746	-	420	WP_012598595.1	DUF202 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (11-51)

Antitoxin

• mRNA interferase PemK-like (4-105)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9913.22 Da
• Isoelectric Point: 4.8359

>T23294 WP_012598589.1 NC_011729:1305929-1306189 [Gloeothece citriformis PCC 7424]
MSTAIRTKIIKIGNSQGIRIPKPLLEQSGIDSEVEIEVQNDYLIVRAVKRSRIGWDEAFAKMAQRGDDALLDEVSTTTWD
HREWEW

Nucleotide

• Length: 261 bp

>T23294 NC_011729:1305929-1306189 [Gloeothece citriformis PCC 7424]
ATGAGTACAGCCATCAGAACTAAGATTATAAAAATAGGCAATTCTCAAGGTATTCGGATTCCTAAACCTCTCCTAGAACA
AAGCGGAATTGATAGCGAGGTGGAAATTGAAGTACAAAATGATTATTTAATAGTTCGTGCAGTAAAGCGATCGCGTATAG
GATGGGATGAAGCTTTTGCAAAAATGGCACAGCGAGGAGATGACGCATTACTCGATGAAGTCAGCACAACAACCTGGGAT
CACAGGGAATGGGAATGGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12079.17 Da
• Isoelectric Point: 6.9203

>AT23294 WP_012598590.1 NC_011729:1306183-1306506 [Gloeothece citriformis PCC 7424]
MVIQRFDVFLVNLAPTVGSEIQKTRPCVIISPDEMNHYIATVIVAPMTTKGQNYPTRIACYCQGLESQIVLDQIRTVDKT
RLVKKLGQISLEEQTAVVETLMNMFAY

Nucleotide

• Length: 324 bp

>AT23294 NC_011729:1306183-1306506 [Gloeothece citriformis PCC 7424]
ATGGTAATTCAACGTTTTGATGTATTTTTAGTTAATCTTGCTCCGACAGTTGGCAGTGAAATTCAAAAAACAAGACCTTG
TGTTATTATTTCCCCAGATGAAATGAATCATTATATTGCCACTGTTATTGTTGCTCCTATGACCACAAAAGGTCAAAACT
ACCCTACTCGTATAGCTTGTTATTGTCAAGGGTTAGAGAGCCAAATAGTTTTAGATCAAATTCGTACAGTTGACAAGACT
CGATTAGTTAAAAAGCTTGGTCAAATTAGCCTAGAAGAACAAACAGCCGTTGTGGAAACTTTGATGAATATGTTTGCTTA
TTAG

Structures

Toxin

• AlphaFold DB: B7K775