Detailed information of TA system TA232937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_26(antitoxin)
• Location: 2311865..2312560
• Replicon: chromosome
• Accession: NZ_CP091227
• Organism: Enterococcus faecalis strain 661

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: E2YXR2
• Locus tag: LQ066_RS11185
• Protein ID: WP_002380839.1
• Coordinates: 2312216..2312560 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: LQ066_RS11180
• Protein ID: WP_002373919.1
• Coordinates: 2311865..2312197 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ066_RS11135 (2307627)	2307627..2308442	-	816	WP_010730098.1	PD-(D/E)XK nuclease-like domain-containing protein	-
LQ066_RS11140 (2308405)	2308405..2309421	-	1017	WP_041161741.1	RecT family recombinase	-
LQ066_RS11145 (2309514)	2309514..2309870	-	357	WP_010774955.1	hypothetical protein	-
LQ066_RS11150 (2309873)	2309873..2310097	-	225	WP_002367281.1	hypothetical protein	-
LQ066_RS11155 (2310094)	2310094..2310393	-	300	WP_014862416.1	hypothetical protein	-
LQ066_RS11160 (2310480)	2310480..2310896	-	417	WP_010730101.1	hypothetical protein	-
LQ066_RS11165 (2310897)	2310897..2311067	-	171	WP_002405829.1	hypothetical protein	-
LQ066_RS11170 (2311102)	2311102..2311371	-	270	WP_002367284.1	hypothetical protein	-
LQ066_RS11175 (2311384)	2311384..2311566	-	183	WP_002358110.1	hypothetical protein	-
LQ066_RS11180 (2311865)	2311865..2312197	+	333	WP_002373919.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ066_RS11185 (2312216)	2312216..2312560	+	345	WP_002380839.1	ImmA/IrrE family metallo-endopeptidase	Toxin
LQ066_RS11190 (2312618)	2312618..2313346	+	729	WP_002380840.1	potassium channel family protein	-
LQ066_RS11195 (2313501)	2313501..2314682	+	1182	WP_010784955.1	site-specific integrase	-
LQ066_RS11200 (2314785)	2314785..2314934	-	150	WP_002356321.1	50S ribosomal protein L33	-
LQ066_RS11205 (2315060)	2315060..2317195	-	2136	WP_002359399.1	penicillin-binding protein 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2281240..2314682	33442

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13640.59 Da
• Isoelectric Point: 5.2241

>T232937 WP_002380839.1 NZ_CP091227:2312216-2312560 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMELYKIPAFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T232937 NZ_CP128487:c2637045-2635723 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCACTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACTCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12734.28 Da
• Isoelectric Point: 5.2423

>AT232937 WP_002373919.1 NZ_CP091227:2311865-2312197 [Enterococcus faecalis]
MNTYEIIKELTKRKKMSIRQLEITLGYSNGYFSKWKKVSPNSEGLQKVADYFNVSVDYLLGRTDNTKATDEKNSDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT232937 NZ_CP128487:c2637311-2637045 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0M2A9N7