Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 479516..480087 Replicon chromosome
Accession NZ_CP091224
Organism Enterococcus faecium strain 237

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag LQ068_RS02285 Protein ID WP_002286801.1
Coordinates 479746..480087 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag LQ068_RS02280 Protein ID WP_002323011.1
Coordinates 479516..479746 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ068_RS02255 (LQ068_02255) 474883..476214 + 1332 WP_002330022.1 FAD-containing oxidoreductase -
LQ068_RS02260 (LQ068_02260) 476236..476862 + 627 WP_002286816.1 cysteine hydrolase -
LQ068_RS02265 (LQ068_02265) 477045..477626 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
LQ068_RS02270 (LQ068_02270) 478111..478686 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
LQ068_RS02275 (LQ068_02275) 478891..479229 - 339 WP_002286804.1 hypothetical protein -
LQ068_RS02280 (LQ068_02280) 479516..479746 + 231 WP_002323011.1 hypothetical protein Antitoxin
LQ068_RS02285 (LQ068_02285) 479746..480087 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LQ068_RS02290 (LQ068_02290) 480937..481122 + 186 WP_002304207.1 hypothetical protein -
LQ068_RS02295 (LQ068_02295) 481392..482554 + 1163 WP_086956687.1 IS3 family transposase -
LQ068_RS02300 (LQ068_02300) 482685..482927 + 243 Protein_458 LPXTG cell wall anchor domain-containing protein -
LQ068_RS02305 (LQ068_02305) 483001..483894 + 894 WP_002286772.1 class C sortase -
LQ068_RS02310 (LQ068_02310) 484078..484752 + 675 WP_002286771.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T232931 WP_002286801.1 NZ_CP091224:479746-480087 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T232931 NZ_CP128484:c90768-90298 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATAGCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT232931 WP_002323011.1 NZ_CP091224:479516-479746 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT232931 NZ_CP128484:c91208-90765 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References