Detailed information of TA system TA232931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 479516..480087
• Replicon: chromosome
• Accession: NZ_CP091224
• Organism: Enterococcus faecium strain 237

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3Y2B8
• Locus tag: LQ068_RS02285
• Protein ID: WP_002286801.1
• Coordinates: 479746..480087 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: LQ068_RS02280
• Protein ID: WP_002323011.1
• Coordinates: 479516..479746 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ068_RS02255 (LQ068_02255)	474883..476214	+	1332	WP_002330022.1	FAD-containing oxidoreductase	-
LQ068_RS02260 (LQ068_02260)	476236..476862	+	627	WP_002286816.1	cysteine hydrolase	-
LQ068_RS02265 (LQ068_02265)	477045..477626	+	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
LQ068_RS02270 (LQ068_02270)	478111..478686	+	576	WP_002293673.1	SOS response-associated peptidase family protein	-
LQ068_RS02275 (LQ068_02275)	478891..479229	-	339	WP_002286804.1	hypothetical protein	-
LQ068_RS02280 (LQ068_02280)	479516..479746	+	231	WP_002323011.1	hypothetical protein	Antitoxin
LQ068_RS02285 (LQ068_02285)	479746..480087	+	342	WP_002286801.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LQ068_RS02290 (LQ068_02290)	480937..481122	+	186	WP_002304207.1	hypothetical protein	-
LQ068_RS02295 (LQ068_02295)	481392..482554	+	1163	WP_086956687.1	IS3 family transposase	-
LQ068_RS02300 (LQ068_02300)	482685..482927	+	243	Protein_458	LPXTG cell wall anchor domain-containing protein	-
LQ068_RS02305 (LQ068_02305)	483001..483894	+	894	WP_002286772.1	class C sortase	-
LQ068_RS02310 (LQ068_02310)	484078..484752	+	675	WP_002286771.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13302.68 Da
• Isoelectric Point: 9.9044

>T232931 WP_002286801.1 NZ_CP091224:479746-480087 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T232931 NZ_CP128484:c90768-90298 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATAGCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT232931 WP_002323011.1 NZ_CP091224:479516-479746 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT232931 NZ_CP128484:c91208-90765 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A829FD66

Antitoxin

• AlphaFold DB: A0A828ZZN9