Detailed information of TA system TA232929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 69339..70447
• Replicon: plasmid p642-a
• Accession: NZ_CP091222
• Organism: Enterococcus faecium strain 642

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: LQ060_RS13735
• Protein ID: WP_000233000.1
• Coordinates: 69578..70447 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LQ060_RS13730
• Protein ID: WP_000205227.1
• Coordinates: 69339..69563 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ060_RS13710 (LQ060_13715)	64970..66453	+	1484	Protein_76	ABC-F type ribosomal protection protein Lsa(E)	-
LQ060_RS13715 (LQ060_13720)	66507..67310	+	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LQ060_RS13720 (LQ060_13725)	67619..68901	-	1283	Protein_78	ISL3 family transposase	-
LQ060_RS13725 (LQ060_13730)	68967..69196	+	230	Protein_79	hypothetical protein	-
LQ060_RS13730 (LQ060_13735)	69339..69563	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ060_RS13735 (LQ060_13740)	69578..70447	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
LQ060_RS13740 (LQ060_13745)	70428..71162	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LQ060_RS13745 (LQ060_13750)	71195..72103	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LQ060_RS13750 (LQ060_13755)	72100..72642	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
LQ060_RS13755 (LQ060_13760)	72735..73529	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
LQ060_RS13760 (LQ060_13765)	73805..74377	+	573	WP_202074493.1	HTH domain-containing protein	-
LQ060_RS13765 (LQ060_13770)	74401..74571	+	171	WP_000374053.1	hypothetical protein	-
LQ060_RS13770 (LQ060_13775)	74605..75285	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T232929 WP_000233000.1 NZ_CP091222:69578-70447 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T232929 NZ_CP128484:27051-27476 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAAGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTGGCTTTGTTGAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT232929 WP_000205227.1 NZ_CP091222:69339-69563 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT232929 NZ_CP128484:26824-27054 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Structures

Toxin

• AlphaFold DB: R3JIN1