Detailed information of TA system TA232927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 147495..148603
• Replicon: plasmid p1525-a
• Accession: NZ_CP091218
• Organism: Enterococcus faecium strain 1525

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: LQ058_RS12795
• Protein ID: WP_000233000.1
• Coordinates: 147495..148364 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LQ058_RS12800
• Protein ID: WP_000205227.1
• Coordinates: 148379..148603 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ058_RS12770 (LQ058_12765)	142604..143614	-	1011	Protein_150	DNA topoisomerase	-
LQ058_RS12775 (LQ058_12770)	143766..145085	-	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-
LQ058_RS12780 (LQ058_12775)	145313..145840	-	528	WP_002294507.1	phosphoribosyltransferase family protein	-
LQ058_RS12785 (LQ058_12780)	145884..146747	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LQ058_RS12790 (LQ058_12785)	146780..147514	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LQ058_RS12795 (LQ058_12790)	147495..148364	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
LQ058_RS12800 (LQ058_12795)	148379..148603	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ058_RS12805 (LQ058_12800)	148746..148975	-	230	Protein_157	hypothetical protein	-
LQ058_RS12810 (LQ058_12805)	149041..150323	+	1283	Protein_158	ISL3 family transposase	-
LQ058_RS12815 (LQ058_12810)	150632..151435	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LQ058_RS12820 (LQ058_12815)	151489..152973	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T232927 WP_000233000.1 NZ_CP091218:c148364-147495 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T232927 NZ_CP128477:c1633682-1633422 [Rhizobium sp. SSM4.3]
GTGCGGGACGTTGTTTACAGCAAGGATGCCATTCGTAGCTTGAACCGGATCCCGGCAAACGAGGCGCGACGTATCCGGTC
GAAGGTTCTTCAGTATGCCACCGACCCGGCTTCCCTCGCCAACAATGTCAAAAAGTTACGTGACAGCCGTTACCATCGCC
TGAGGATCGGGGACTGGCGCGTGATCTTTCGTGAAGACGGAACCGTCGTTGACGTGGTGCGTGTCGCAGCACGCGGTGAA
GCCTATGAGGGAGAGCTCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT232927 WP_000205227.1 NZ_CP091218:c148603-148379 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT232927 NZ_CP128477:c1633422-1633012 [Rhizobium sp. SSM4.3]
ATGACGATCGCATCATCGTTTACGACGCCAAATGGCGAAGAGATGATCGTACTCAGCCGCAAGGACTACGAAGCGCTCAT
CGCGCGCATCGAGGATTTGGAAGACAGTCTGGCTGTCGCCGATTTCGAGAGGAAACTTGCTGCGAGCGAGGAGGAACTGA
TCCCTGCCGAGTACGTCAACCGGATGGGTGAAGGTGAAAGCCCGATCCGCGTCTGGCGCGATTTTCGTGGCATGGCGGCC
AAGGACCTTGCTGCTGCTGCCGGAATCAGCACCGCTTATCTGTCCGAGATCGAAACCGGCAAGAAGGACGGCAGTGTCGC
AACACTCAAAGCGATCGCCGATGTCTTGAAGCTCGACCTTGATGACCTCTACTGGCGGCACGAGGACAGGAAGGTCACAG
ATACCGCATGA

Structures

Toxin

• AlphaFold DB: R3JIN1