Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 474833..475404 Replicon chromosome
Accession NZ_CP091217
Organism Enterococcus faecium strain 1525

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag LQ058_RS02245 Protein ID WP_002302307.1
Coordinates 475063..475404 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag LQ058_RS02240 Protein ID WP_002323011.1
Coordinates 474833..475063 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ058_RS02215 (LQ058_02215) 470322..471650 + 1329 WP_010718318.1 FAD-dependent oxidoreductase -
LQ058_RS02220 (LQ058_02220) 471672..472298 + 627 WP_002286816.1 cysteine hydrolase -
LQ058_RS02225 (LQ058_02225) 472481..473062 + 582 WP_043902871.1 TetR/AcrR family transcriptional regulator -
LQ058_RS02230 (LQ058_02230) 473427..474002 + 576 WP_010718317.1 SOS response-associated peptidase family protein -
LQ058_RS02235 (LQ058_02235) 474207..474545 - 339 WP_002286804.1 hypothetical protein -
LQ058_RS02240 (LQ058_02240) 474833..475063 + 231 WP_002323011.1 hypothetical protein Antitoxin
LQ058_RS02245 (LQ058_02245) 475063..475404 + 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LQ058_RS02250 (LQ058_02250) 476254..476439 + 186 WP_002304207.1 hypothetical protein -
LQ058_RS02260 (LQ058_02260) 477958..478200 + 243 Protein_448 LPXTG cell wall anchor domain-containing protein -
LQ058_RS02265 (LQ058_02265) 478274..479167 + 894 WP_002326335.1 class C sortase -
LQ058_RS02270 (LQ058_02270) 479350..480024 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T232926 WP_002302307.1 NZ_CP091217:475063-475404 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T232926 NZ_CP128470:c1822060-1821719 [Macrococcus bovicus]
ATGAGGCGCGGCGAAGTCTTTCTGGCTGACCTGTCACCTGTCAAAGGCTCGGAACAAGGCGGTAAGCGTCCTGTTGTCAT
TATTCAGAATGATGTCGGGAATAAATACAGTCCGACAGTCATCGTGGCTGCAATCACAGCGAAAATCAATAAAGCACGGA
TCCCGACCCATGTGGAGATCGGCAAGACCAACCATCATCTGGATAAGGATTCGGTGATATTGCTTGAACAGATCAGAACG
ATTGATAAAAATCGACTGATTGAAAAGCTGACAGTTCTCTCTGAAGAAAAAATGAGTGAAGTGAATCAGGCGCTGGCCAT
CAGCCTTGGACTCTCTCAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT232926 WP_002323011.1 NZ_CP091217:474833-475063 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT232926 NZ_CP128470:c1822204-1822061 [Macrococcus bovicus]
ATGAAGAAATTTGATCAATTATTAGCTGAAGGCTATGAATCCATGGCTGAACTTAATTTAATCATTTCTTATGAGCAATT
CGATCTTGAAAGTGAAGTGTGTGAAAAGAGTGAAATGTACGATTTAGCTGATGATGGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References