Detailed information of TA system TA232924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 64936..66044
• Replicon: plasmid p264a-a
• Accession: NZ_CP091214
• Organism: Enterococcus faecium strain 264a

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: -
• Locus tag: LQ059_RS12665
• Protein ID: WP_002328890.1
• Coordinates: 65175..66044 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LQ059_RS12660
• Protein ID: WP_000205227.1
• Coordinates: 64936..65160 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ059_RS12645 (LQ059_12645)	60567..62051	+	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-
LQ059_RS12650 (LQ059_12650)	62105..62908	+	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LQ059_RS12655 (LQ059_12655)	63217..64518	-	1302	Protein_74	ISL3 family transposase	-
LQ059_RS12660 (LQ059_12660)	64936..65160	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ059_RS12665 (LQ059_12665)	65175..66044	+	870	WP_002328890.1	nucleotidyltransferase domain-containing protein	Toxin
LQ059_RS12670 (LQ059_12670)	66025..66759	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LQ059_RS12675 (LQ059_12675)	66792..67700	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LQ059_RS12680 (LQ059_12680)	67697..68239	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
LQ059_RS12685 (LQ059_12685)	68332..69126	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
LQ059_RS13685	69536..69619	+	84	Protein_81	MLS leader peptide	-
LQ059_RS12690 (LQ059_12690)	69668..69763	+	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
LQ059_RS12695 (LQ059_12695)	69888..70625	+	738	WP_002321849.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32888.48 Da
• Isoelectric Point: 4.8759

>T232924 WP_002328890.1 NZ_CP091214:65175-66044 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T232924 NZ_CP128466:c5431178-5430795 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT232924 WP_000205227.1 NZ_CP091214:64936-65160 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT232924 NZ_CP128466:c5431420-5431178 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTTCGCAAGGAGTGG
TAA