Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 467050..467621 Replicon chromosome
Accession NZ_CP091210
Organism Enterococcus faecium strain 211b

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag LQ064_RS02215 Protein ID WP_002302307.1
Coordinates 467280..467621 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag LQ064_RS02210 Protein ID WP_002323011.1
Coordinates 467050..467280 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ064_RS02180 (LQ064_02180) 462822..463448 + 627 WP_002286816.1 cysteine hydrolase -
LQ064_RS02185 (LQ064_02185) 463631..464212 + 582 WP_043902871.1 TetR/AcrR family transcriptional regulator -
LQ064_RS02190 (LQ064_02190) 464577..464949 + 373 Protein_434 SOS response-associated peptidase family protein -
LQ064_RS02195 (LQ064_02195) 465022..465981 + 960 WP_000222577.1 IS30-like element IS1252 family transposase -
LQ064_RS02200 (LQ064_02200) 466001..466219 + 219 WP_283255635.1 SOS response-associated peptidase family protein -
LQ064_RS02205 (LQ064_02205) 466424..466762 - 339 WP_002286804.1 hypothetical protein -
LQ064_RS02210 (LQ064_02210) 467050..467280 + 231 WP_002323011.1 hypothetical protein Antitoxin
LQ064_RS02215 (LQ064_02215) 467280..467621 + 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LQ064_RS02220 (LQ064_02220) 468471..468656 + 186 WP_002304207.1 hypothetical protein -
LQ064_RS02230 (LQ064_02230) 470175..470417 + 243 Protein_442 LPXTG cell wall anchor domain-containing protein -
LQ064_RS02235 (LQ064_02235) 470491..471384 + 894 WP_002326335.1 class C sortase -
LQ064_RS02240 (LQ064_02240) 471567..472241 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 465022..470097 5075


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T232921 WP_002302307.1 NZ_CP091210:467280-467621 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T232921 NZ_CP128466:c4829890-4829603 [Klebsiella variicola]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCATTAAGTAATCTACGAAAACATGGAGTCCGGTTTGAAGAAGCGGT
ATTAGTCTTTGACGATCCGCGGCACCTTTCCCGCCAGGAGCGGTTTGAGAATGGTGAATATCGCTGGCAGACCATCGGTC
TTGTGCATGGGATCCTTGTTATCCTGGTGGCGCATTCGGTCCGTTTTGAAAGTGGAACTGAGGTTATTCGTATTATCAGC
GCCCGGAAGGCGGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT232921 WP_002323011.1 NZ_CP091210:467050-467280 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT232921 NZ_CP128466:c4829616-4829314 [Klebsiella variicola]
ATGAGCATGGTTAAACATAAACGCGGCAATACGGCGGCGCTTACCGCTAAGCGTGATGCCGAACTCAAAGCGCTAGCGAG
CAAAGCCGATGAGGATATCGATTACAGCGATATCCCCGCTACAGACGATGCGCAATGGTCAGATGCCGTTCGCGGCAAAT
TTTTTCGACCGCTAAAAACACAGGCTTCTGTGCGCATTGATGCCGATGTCATGGAGTGGCTCAAACGTCCAGGAAAAGGC
TATCAAACTCGACTGAATGCGATTTTAAGAGAAGCGATGATTCGCGATCTTAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References