Detailed information of TA system TA023292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-AbrB
• Location: 3805025..3805613
• Replicon: chromosome
• Accession: NC_011726
• Organism: Cyanothece sp. PCC 8801

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B7K1R7
• Locus tag: PCC8801_RS17755
• Protein ID: WP_012596867.1
• Coordinates: 3805025..3805366 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B7K1R8
• Locus tag: PCC8801_RS17760
• Protein ID: WP_012596868.1
• Coordinates: 3805353..3805613 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCC8801_RS17735	3800311..3801531	+	1221	WP_012596863.1	tetratricopeptide repeat protein	-
PCC8801_RS23660	3801552..3801725	+	174	WP_012596864.1	hypothetical protein	-
PCC8801_RS17745	3801864..3803150	-	1287	WP_012596865.1	glutamyl-tRNA reductase	-
PCC8801_RS17750	3803498..3804535	-	1038	WP_012596866.1	class II fructose-bisphosphatase	-
PCC8801_RS17755	3805025..3805366	-	342	WP_012596867.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PCC8801_RS17760	3805353..3805613	-	261	WP_012596868.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-110)

Antitoxin

• SpoVT-AbrB domain (17-59)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12634.04 Da
• Isoelectric Point: 10.7397

>T23292 WP_012596867.1 NC_011726:c3805366-3805025 [Rippkaea orientalis PCC 8801]
MVTYNQFDVVVVPFPLTDKTTSKKRPALVISDMAFNLSLKKIVMAMITTSGHSSWMFDVSIVNLAASGLKSPSLIRMKLF
TLDDALIVRKIGTLTQSDQERVKNSLKQLFKLL

Nucleotide

• Length: 342 bp

>T23292 NC_011726:c3805366-3805025 [Rippkaea orientalis PCC 8801]
ATGGTGACTTATAACCAGTTTGATGTTGTAGTTGTCCCTTTTCCCTTGACTGATAAAACAACATCTAAAAAACGTCCTGC
ATTAGTTATTTCTGATATGGCTTTTAATTTATCCTTAAAAAAGATTGTTATGGCTATGATTACTACCAGTGGTCATTCTT
CTTGGATGTTTGATGTTTCTATTGTTAATTTAGCTGCGTCGGGGCTTAAATCTCCATCCTTAATTAGAATGAAGTTATTT
ACTCTAGATGATGCTTTGATTGTAAGGAAAATCGGCACATTGACGCAATCAGATCAAGAGAGGGTCAAAAATTCACTAAA
ACAGTTATTTAAGTTGTTGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9898.16 Da
• Isoelectric Point: 4.3054

>AT23292 WP_012596868.1 NC_011726:c3805613-3805353 [Rippkaea orientalis PCC 8801]
MQNQSAQSKCDNITVSKLTQKYQATIPRRIREVLDLHQGDSVIFELQDDKVILKKVLPMDWDYLNAVSATLTEWLSPEDE
EAYGDL

Nucleotide

• Length: 261 bp

>AT23292 NC_011726:c3805613-3805353 [Rippkaea orientalis PCC 8801]
ATGCAAAACCAATCAGCTCAATCTAAGTGCGATAACATAACAGTCTCTAAACTCACCCAAAAATACCAGGCGACCATTCC
ACGGCGTATAAGGGAAGTTTTAGACTTACATCAAGGAGATTCAGTTATTTTTGAGTTGCAAGATGATAAGGTAATTTTGA
AGAAAGTGCTACCGATGGATTGGGACTATCTCAATGCAGTGTCAGCAACACTAACTGAGTGGCTATCTCCTGAAGATGAG
GAAGCCTATGGTGACTTATAA

Structures

Toxin

• AlphaFold DB: B7K1R7

Antitoxin

• AlphaFold DB: B7K1R8