Detailed information of TA system TA232919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 12700..13837
• Replicon: plasmid p1818-b
• Accession: NZ_CP091208
• Organism: Enterococcus faecium strain 1818

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: LQ061_RS12555
• Protein ID: WP_002333003.1
• Coordinates: 12700..13563 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: B3CKC7
• Locus tag: LQ061_RS12560
• Protein ID: WP_002333002.1
• Coordinates: 13565..13837 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ061_RS12530 (LQ061_12530)	7924..8499	+	576	WP_033603447.1	IS30 family transposase	-
LQ061_RS12535 (LQ061_12535)	8571..10184	-	1614	WP_002333004.1	hypothetical protein	-
LQ061_RS12540 (LQ061_12540)	10208..11089	-	882	WP_002326774.1	ABC transporter ATP-binding protein	-
LQ061_RS12545 (LQ061_12545)	11400..11996	+	597	WP_002326773.1	TetR/AcrR family transcriptional regulator	-
LQ061_RS12550 (LQ061_12550)	12165..12569	-	405	Protein_13	DnaJ domain-containing protein	-
LQ061_RS12555 (LQ061_12555)	12700..13563	-	864	WP_002333003.1	zeta toxin family protein	Toxin
LQ061_RS12560 (LQ061_12560)	13565..13837	-	273	WP_002333002.1	antitoxin	Antitoxin
LQ061_RS12565 (LQ061_12565)	13855..14070	-	216	WP_001835296.1	peptide-binding protein	-
LQ061_RS12570 (LQ061_12570)	14162..15058	-	897	WP_104681373.1	ParA family protein	-
LQ061_RS12575 (LQ061_12575)	15161..15985	-	825	Protein_18	DNA topoisomerase	-
LQ061_RS12580 (LQ061_12580)	15980..16186	+	207	WP_001281297.1	protein rep	-
LQ061_RS12585 (LQ061_12585)	16386..17762	+	1377	WP_001574277.1	tetracycline efflux MFS transporter Tet(L)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(S) / tet(L)	-	1..36787	36787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32365.76 Da
• Isoelectric Point: 6.3643

>T232919 WP_002333003.1 NZ_CP091208:c13563-12700 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVVVIDNDTFKQQHPNFDELV
KLYEKDVVKHATPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKTYAMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T232919 NZ_CP128466:c4049688-4049371 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
ATCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.29 Da
• Isoelectric Point: 7.1987

>AT232919 WP_002333002.1 NZ_CP091208:c13837-13565 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELKAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT232919 NZ_CP128466:c4049378-4049091 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A2Z6BWJ3