Detailed information of TA system TA232918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 41217..41834
• Replicon: plasmid p1818-a
• Accession: NZ_CP091207
• Organism: Enterococcus faecium strain 1818

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LQ061_RS12015
• Protein ID: WP_231410992.1
• Coordinates: 41217..41369 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LQ061_RS12020
• Protein ID: WP_047937794.1
• Coordinates: 41403..41834 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ061_RS11975 (LQ061_11985)	36440..37255	+	816	WP_231410993.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
LQ061_RS11980 (LQ061_11990)	37258..37743	+	486	WP_002318321.1	single-stranded DNA-binding protein	-
LQ061_RS11985 (LQ061_11995)	38109..38369	+	261	WP_002293538.1	hypothetical protein	-
LQ061_RS11990 (LQ061_12000)	38814..39020	+	207	WP_002325537.1	hypothetical protein	-
LQ061_RS11995 (LQ061_12005)	39020..39271	+	252	WP_212481448.1	hypothetical protein	-
LQ061_RS12000 (LQ061_12010)	39287..39724	+	438	WP_249489256.1	hypothetical protein	-
LQ061_RS12005 (LQ061_12015)	39717..40425	+	709	Protein_41	hypothetical protein	-
LQ061_RS12010 (LQ061_12020)	40913..41092	+	180	WP_002295613.1	hypothetical protein	-
LQ061_RS12015 (LQ061_12025)	41217..41369	+	153	WP_231410992.1	type II toxin-antitoxin system HicA family toxin	Toxin
LQ061_RS12020 (LQ061_12030)	41403..41834	+	432	WP_047937794.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LQ061_RS12025 (LQ061_12035)	42065..43024	-	960	WP_212481452.1	IS30 family transposase	-
LQ061_RS12030 (LQ061_12040)	43134..43961	+	828	Protein_46	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..138406	138406
	-	flank	IS/Tn	-	-	42065..43024	959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5727.53 Da
• Isoelectric Point: 10.2749

>T232918 WP_231410992.1 NZ_CP091207:41217-41369 [Enterococcus faecium]
IEKNGWVERRQEGSHHHLYKDGVRITVPVHANQDLGRGLEKKILKDAGLK

Nucleotide

• Length: 153 bp

>T232918 NZ_CP128466:c2677402-2676080 [Klebsiella variicola]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGCACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCT
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTACCCGACAGCCCGCAGGTCCGCGATCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTGGGACGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCACCTTGAGGGGTTGCGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCCTACCAGT
CGGATATCCCTCTCGGGATGATTACCGGCCAGGATGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGGGCACTGGTGTATTCCGCAGGGCACCACGCCGACCACGCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCTAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCGCTGGCCAGGGAACTGG
GCCTGGCGGTGCCGGAGGCAGAGATTATCACCACGCCGCGGATCCGCGCGCTGGCGGTGACCCGCTTTGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTACTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCCTTTGGCATCCCGTCGTCGATGAAATA
TGAATCTGACGGCGGGCCGGGTATTGCGGCAATCATGGCCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCGCAG
ACTTTATGAAATTCATGGTTTTTCAGTGGCTGGTTGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGTGGCAGCTACCGTCTAACGCCGTTCTACGACATCATTTCGGCGTTTCCGGTGCTGGGAGGCACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCAACCGCCAAAGCGGTGAACTTTCCTGGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCGGGCCATTACGGAAAATATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15840.92 Da
• Isoelectric Point: 4.0728

>AT232918 WP_047937794.1 NZ_CP091207:41403-41834 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEELLPEPTPINKISVEDD
SFTTLIRVDVAEYLKETELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT232918 NZ_CP128466:c2677668-2677402 [Klebsiella variicola]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTATCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACACTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTAGGGGTGGTGATGACGCTGAACGCCAAAGCCACAGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA