Detailed information of TA system TA232916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 711582..712153
• Replicon: chromosome
• Accession: NZ_CP091206
• Organism: Enterococcus faecium strain 1818

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LQ061_RS03415
• Protein ID: WP_002327666.1
• Coordinates: 711582..711923 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: LQ061_RS03420
• Protein ID: WP_002323011.1
• Coordinates: 711923..712153 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ061_RS03395 (LQ061_03400)	707437..708462	-	1026	WP_002286770.1	sensor histidine kinase	-
LQ061_RS03400 (LQ061_03405)	708480..709154	-	675	WP_002291233.1	response regulator transcription factor	-
LQ061_RS03405 (LQ061_03410)	709335..709529	-	195	Protein_673	class C sortase	-
LQ061_RS03410 (LQ061_03415)	709603..710765	+	1163	WP_154081291.1	IS3 family transposase	-
LQ061_RS03415 (LQ061_03420)	711582..711923	-	342	WP_002327666.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LQ061_RS03420 (LQ061_03425)	711923..712153	-	231	WP_002323011.1	hypothetical protein	Antitoxin
LQ061_RS03425 (LQ061_03430)	712449..712787	+	339	WP_002327665.1	hypothetical protein	-
LQ061_RS03430 (LQ061_03435)	712988..713563	-	576	WP_249489158.1	SOS response-associated peptidase family protein	-
LQ061_RS03435 (LQ061_03440)	714295..714876	-	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
LQ061_RS03440 (LQ061_03445)	715059..715685	-	627	WP_002286816.1	cysteine hydrolase	-
LQ061_RS03445 (LQ061_03450)	715707..717035	-	1329	WP_249489159.1	FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13264.67 Da
• Isoelectric Point: 10.1461

>T232916 WP_002327666.1 NZ_CP091206:c711923-711582 [Enterococcus faecium]
VSGERIYIPKKGDIVWIYFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T232916 NZ_CP128466:1797524-1797856 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACTCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT232916 WP_002323011.1 NZ_CP091206:c712153-711923 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT232916 NZ_CP128466:1797266-1797523 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A828ZZN9