Detailed information of TA system TA232914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 3025567..3026675
• Replicon: chromosome
• Accession: NZ_CP091204
• Organism: Enterococcus gallinarum strain 674

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: -
• Locus tag: LQ057_RS14635
• Protein ID: WP_078126936.1
• Coordinates: 3025567..3026436 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: B9DSK1
• Locus tag: LQ057_RS14640
• Protein ID: WP_002303393.1
• Coordinates: 3026451..3026675 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ057_RS14610 (LQ057_14610)	3020996..3021451	+	456	Protein_2854	transposase	-
LQ057_RS14615 (LQ057_14615)	3021838..3023157	-	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-
LQ057_RS14620 (LQ057_14620)	3023385..3023912	-	528	WP_002294507.1	phosphoribosyltransferase family protein	-
LQ057_RS14625 (LQ057_14625)	3023956..3024819	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LQ057_RS14630 (LQ057_14630)	3024852..3025586	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LQ057_RS14635 (LQ057_14635)	3025567..3026436	-	870	WP_078126936.1	nucleotidyltransferase domain-containing protein	Toxin
LQ057_RS14640 (LQ057_14640)	3026451..3026675	-	225	WP_002303393.1	helix-turn-helix transcriptional regulator	Antitoxin
LQ057_RS14645 (LQ057_14645)	3026818..3027639	-	822	Protein_2861	recombinase zinc beta ribbon domain-containing protein	-
LQ057_RS14650 (LQ057_14650)	3028327..3029130	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LQ057_RS14655 (LQ057_14655)	3029184..3030668	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32835.43 Da
• Isoelectric Point: 4.7269

>T232914 WP_078126936.1 NZ_CP091204:c3026436-3025567 [Enterococcus gallinarum]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T232914 NZ_CP128466:341944-342312 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGACGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGTGATTTCTCGCGGCCATATTTTCAATGATGGAAATAAGCGTACGGCACTGTTT
ATTACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCGGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8351.65 Da
• Isoelectric Point: 4.5357

>AT232914 WP_002303393.1 NZ_CP091204:c3026675-3026451 [Enterococcus gallinarum]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Nucleotide

• Length: 225 bp

>AT232914 NZ_CP128466:341726-341947 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1M5SHM7