Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 13192..14329 Replicon plasmid p697-a
Accession NZ_CP091202
Organism Enterococcus hirae strain 697

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag LQ063_RS12910 Protein ID WP_002332783.1
Coordinates 13466..14329 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag LQ063_RS12905 Protein ID WP_002326825.1
Coordinates 13192..13464 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ063_RS12880 (LQ063_12885) 8607..10091 + 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -
LQ063_RS12885 (LQ063_12890) 10145..10948 + 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
LQ063_RS12890 (LQ063_12895) 11623..11868 + 246 Protein_15 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
LQ063_RS12895 (LQ063_12900) 11971..12867 + 897 WP_193566075.1 ParA family protein -
LQ063_RS12900 (LQ063_12905) 12959..13174 + 216 WP_001835296.1 peptide-binding protein -
LQ063_RS12905 (LQ063_12910) 13192..13464 + 273 WP_002326825.1 antitoxin Antitoxin
LQ063_RS12910 (LQ063_12915) 13466..14329 + 864 WP_002332783.1 zeta toxin family protein Toxin
LQ063_RS12915 (LQ063_12920) 14769..15086 + 318 WP_002326830.1 hypothetical protein -
LQ063_RS12920 (LQ063_12925) 15620..16117 + 498 WP_002325587.1 hypothetical protein -
LQ063_RS12925 (LQ063_12930) 16304..16984 + 681 WP_000191454.1 IS6-like element ISEnfa1 family transposase -
LQ063_RS12930 (LQ063_12935) 17181..18011 - 831 WP_000242080.1 undecaprenyl-diphosphate phosphatase -
LQ063_RS12935 (LQ063_12940) 18011..18712 - 702 WP_002390458.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid erm(B) / ant(6)-Ia / lsa(E) / lnu(B) / tet(L) / tet(M) / poxtA / fexB - 1..53013 53013


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T232913 WP_002332783.1 NZ_CP091202:13466-14329 [Enterococcus hirae]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T232913 NZ_CP128464:2770331-2770474 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT232913 WP_002326825.1 NZ_CP091202:13192-13464 [Enterococcus hirae]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT232913 NZ_CP128464:c2770668-2770406 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATATCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTTTTCTTTTACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References