Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 26701..27235 Replicon plasmid pUK045_2
Accession NZ_CP091199
Organism Enterococcus faecalis strain UK045

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag L2629_RS17505 Protein ID WP_002360769.1
Coordinates 26960..27235 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag L2629_RS17500 Protein ID WP_002369771.1
Coordinates 26701..26967 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2629_RS17465 (L2629_17405) 22926..23936 + 1011 WP_010783124.1 replication initiator protein A -
L2629_RS17470 (L2629_17410) 23980..24459 + 480 WP_236335509.1 TraB domain-containing protein -
L2629_RS17475 (L2629_17415) 24492..24992 + 501 WP_002360775.1 DnaJ domain-containing protein -
L2629_RS17480 (L2629_17420) 25005..25253 + 249 WP_002417063.1 DUF3850 domain-containing protein -
L2629_RS17485 (L2629_17425) 25329..25745 + 417 WP_002368093.1 single-stranded DNA-binding protein -
L2629_RS17490 (L2629_17430) 25769..26353 + 585 WP_002369774.1 thermonuclease family protein -
L2629_RS17495 (L2629_17435) 26350..26598 + 249 WP_002369773.1 hypothetical protein -
L2629_RS17500 (L2629_17440) 26701..26967 + 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
L2629_RS17505 (L2629_17445) 26960..27235 + 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
L2629_RS17510 (L2629_17450) 28038..28223 + 186 WP_002365983.1 hypothetical protein -
L2629_RS17515 (L2629_17455) 28208..28399 + 192 WP_002399282.1 DpnD/PcfM family protein -
L2629_RS17520 (L2629_17460) 28423..29013 + 591 WP_002360842.1 hypothetical protein -
L2629_RS17525 (L2629_17465) 29118..29897 + 780 WP_002390215.1 Cas9 inhibitor AcrIIA9 family protein -
L2629_RS17530 (L2629_17470) 29897..31240 + 1344 WP_002417363.1 PcfJ domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) - 1..41202 41202


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-87)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T232912 WP_002360769.1 NZ_CP091199:26960-27235 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T232912 NZ_CP128464:2770331-2770474 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9804.15 Da        Isoelectric Point: 4.4791

>AT232912 WP_002369771.1 NZ_CP091199:26701-26967 [Enterococcus faecalis]
MATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT232912 NZ_CP128464:c2770234-2770033 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References