Detailed information of TA system TA232906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 1339736..1340753
• Replicon: chromosome
• Accession: NZ_CP091198
• Organism: Enterococcus faecalis strain UK045

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: Q839N5
• Locus tag: L2629_RS07240
• Protein ID: WP_002359283.1
• Coordinates: 1340115..1340753 (+)
• Length: 213 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: L2629_RS07235
• Protein ID: WP_236335383.1
• Coordinates: 1339736..1340104 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2629_RS07190 (1335677)	1335677..1336060	-	384	WP_002359294.1	DUF86 domain-containing protein	-
L2629_RS07195 (1336050)	1336050..1336382	-	333	WP_002359293.1	nucleotidyltransferase domain-containing protein	-
L2629_RS07200 (1336494)	1336494..1336898	-	405	WP_002359292.1	hypothetical protein	-
L2629_RS07205 (1336880)	1336880..1337071	-	192	WP_010773668.1	hypothetical protein	-
L2629_RS07210 (1337100)	1337100..1337324	-	225	WP_002359290.1	hypothetical protein	-
L2629_RS07215 (1337474)	1337474..1337881	-	408	WP_002359289.1	DUF961 family protein	-
L2629_RS07220 (1337881)	1337881..1338207	-	327	WP_002359288.1	hypothetical protein	-
L2629_RS07225 (1338294)	1338294..1338545	-	252	WP_002359287.1	hypothetical protein	-
L2629_RS07230 (1338712)	1338712..1338894	+	183	WP_002359286.1	hypothetical protein	-
L2629_RS07235 (1339736)	1339736..1340104	+	369	WP_236335383.1	helix-turn-helix domain-containing protein	Antitoxin
L2629_RS07240 (1340115)	1340115..1340753	+	639	WP_002359283.1	ImmA/IrrE family metallo-endopeptidase	Toxin
L2629_RS07245 (1340795)	1340795..1341370	-	576	WP_002359282.1	restriction endonuclease	-
L2629_RS07250 (1341966)	1341966..1343156	-	1191	WP_236335389.1	SIR2 family protein	-
L2629_RS07255 (1343577)	1343577..1343879	-	303	WP_002387498.1	hypothetical protein	-
L2629_RS07260 (1343968)	1343968..1344186	+	219	WP_025187569.1	helix-turn-helix transcriptional regulator	-
L2629_RS07265 (1344237)	1344237..1344584	-	348	WP_002387496.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	tufA / EF0149	1270005..1368729	98724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (86-176)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-61)

Sequences

Toxin

Protein

• Length: 213 a.a.
• Molecular weight: 25186.21 Da
• Isoelectric Point: 9.1676

>T232906 WP_002359283.1 NZ_CP091198:1340115-1340753 [Enterococcus faecalis]
MILNKKKLYFETQAFTDKLINEISINMSKRKELINCYDIEAYVKETENIDFFDYPFKKQSKKLIWGSVTKIGDVTMVATA
QHLKIEIKNFTKMHEIIHHYFDYTTDMPNNEKSFDTLLIKKGYLPEDYPKEFRANKGAGMLLINKKALKYAFSTYDDLEK
MASFFNVNDIVLKIRILDELIYSHNLSYKQANLIYNSYYFQQQTQLKEILLK

Nucleotide

• Length: 639 bp

>T232906 NZ_CP128464:c2607902-2607801 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14303.04 Da
• Isoelectric Point: 4.7452

>AT232906 WP_236335383.1 NZ_CP091198:1339736-1340104 [Enterococcus faecalis]
MKTYEKIKQLRENREITQKEISNALDINVSVYNKIELGIRPLREEELTAIADFFNVSIDYLTGRTDNPTTPSNNQKEAGQ
NIISHFRLNTNDLSSDDIEELEKELIDFQEFLIKRAKERKKE

Nucleotide

• Length: 369 bp

>AT232906 NZ_CP128464:2607677-2607856 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAGTGCAACAA

Structures

Toxin

• AlphaFold DB: A0A059N6D0