Detailed information of TA system TA023289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 1498857..1499497
• Replicon: chromosome
• Accession: NC_011726
• Organism: Cyanothece sp. PCC 8801

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B7K4M0
• Locus tag: PCC8801_RS06950
• Protein ID: WP_012594758.1
• Coordinates: 1498857..1499255 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7K4M1
• Locus tag: PCC8801_RS06955
• Protein ID: WP_012594759.1
• Coordinates: 1499252..1499497 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCC8801_RS06930	1495238..1495831	-	594	WP_012594754.1	hormogonium polysaccharide biosynthesis acetyltransferase HpsU	-
PCC8801_RS06935	1495928..1496860	-	933	WP_012594755.1	glycosyltransferase family 2 protein	-
PCC8801_RS06940	1497073..1498422	-	1350	WP_012594756.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
PCC8801_RS06945	1498483..1498731	-	249	WP_012594757.1	hypothetical protein	-
PCC8801_RS06950	1498857..1499255	-	399	WP_012594758.1	type II toxin-antitoxin system VapC family toxin	Toxin
PCC8801_RS06955	1499252..1499497	-	246	WP_012594759.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PCC8801_RS06960	1499863..1500546	+	684	WP_012594760.1	toll/interleukin-1 receptor domain-containing protein	-
PCC8801_RS06965	1500682..1502559	-	1878	WP_012594761.1	diguanylate cyclase	-
PCC8801_RS06970	1502812..1503183	-	372	WP_012594762.1	heterocyst frequency control protein PatD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (4-128)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-42)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15042.30 Da
• Isoelectric Point: 5.1199

>T23289 WP_012594758.1 NC_011726:c1499255-1498857 [Rippkaea orientalis PCC 8801]
VRLLLDTQCWLWWFAQPEKLSENVIEQIANESNEVWFSVASVWEMGIKVSIGKLPLPEPIDDYVSTRMTQLGARSLKINA
SHALRVAALPLHHRDPFDRMLIAQAQVEDMTLVSADSTFNQYKVSLLWAASF

Nucleotide

• Length: 399 bp

>T23289 NC_011726:c1499255-1498857 [Rippkaea orientalis PCC 8801]
GTGAGACTTTTACTAGATACACAATGCTGGCTGTGGTGGTTTGCTCAACCAGAAAAGTTAAGTGAGAATGTAATTGAACA
AATTGCCAACGAAAGCAATGAAGTGTGGTTTTCTGTTGCCAGTGTTTGGGAGATGGGAATTAAGGTTTCAATCGGTAAGC
TGCCACTGCCAGAACCAATAGATGATTACGTTTCTACTCGAATGACTCAACTAGGAGCTAGATCGTTAAAGATCAATGCT
TCTCATGCTTTGCGGGTGGCTGCATTGCCTTTACATCATCGTGATCCTTTTGACCGAATGCTGATTGCACAAGCTCAGGT
AGAAGATATGACACTTGTGAGTGCGGATTCAACATTTAATCAATATAAAGTCTCTTTGCTTTGGGCAGCTAGTTTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8924.16 Da
• Isoelectric Point: 5.0190

>AT23289 WP_012594759.1 NC_011726:c1499497-1499252 [Rippkaea orientalis PCC 8801]
METVNIHQAKTNLSRLLSRVELGEVIVISNRGIPIAKLVPFRTSSDRRSSLGQDRGMFTVPDDFNAPLPEDILVAFEGGT
E

Nucleotide

• Length: 246 bp

>AT23289 NC_011726:c1499497-1499252 [Rippkaea orientalis PCC 8801]
ATGGAAACAGTAAACATTCATCAGGCTAAAACAAACCTTTCTCGGCTATTGTCCCGTGTGGAACTCGGAGAAGTAATCGT
TATTTCAAACCGAGGTATCCCCATTGCTAAGCTTGTTCCATTTCGGACATCTTCAGATCGACGGTCTAGTTTAGGACAAG
ATCGAGGGATGTTTACCGTACCAGATGACTTTAATGCTCCTTTACCAGAAGATATTTTGGTAGCATTTGAGGGTGGCACA
GAGTGA

Structures

Toxin

• AlphaFold DB: B7K4M0

Antitoxin

• AlphaFold DB: B7K4M1