Detailed information of TA system TA023288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1247745..1248325
• Replicon: chromosome
• Accession: NC_011726
• Organism: Cyanothece sp. PCC 8801

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B7K2D0
• Locus tag: PCC8801_RS05855
• Protein ID: WP_012594540.1
• Coordinates: 1247745..1248089 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B7K2D1
• Locus tag: PCC8801_RS05860
• Protein ID: WP_012594541.1
• Coordinates: 1248083..1248325 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCC8801_RS05835	1243066..1243884	-	819	WP_012594536.1	carbon-nitrogen hydrolase family protein	-
PCC8801_RS05840	1243968..1245134	-	1167	WP_012594537.1	pyridoxal phosphate-dependent aminotransferase	-
PCC8801_RS05845	1245238..1246947	-	1710	WP_012594538.1	mechanosensitive ion channel family protein	-
PCC8801_RS05850	1247033..1247686	-	654	WP_012594539.1	Uma2 family endonuclease	-
PCC8801_RS05855	1247745..1248089	-	345	WP_012594540.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PCC8801_RS05860	1248083..1248325	-	243	WP_012594541.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PCC8801_RS05865	1248584..1250299	-	1716	WP_203427711.1	AAA family ATPase	-
PCC8801_RS05870	1250417..1251514	-	1098	WP_012594543.1	4Fe-4S ferredoxin	-
PCC8801_RS05875	1251608..1252450	-	843	WP_012594544.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• SpoVT-AbrB domain (8-49)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12666.87 Da
• Isoelectric Point: 4.8010

>T23288 WP_012594540.1 NC_011726:c1248089-1247745 [Rippkaea orientalis PCC 8801]
MVTIGDRVPERGDIIRLELNPPTGSEQAGYRPALIISPVAYNRISKLILICPITSRQKGWPFEVLLPPSLQTYGVVLVDQ
IRSIDCQARKADFVEILPADVMDEVLAKLEPLLT

Nucleotide

• Length: 345 bp

>T23288 NC_011726:c1248089-1247745 [Rippkaea orientalis PCC 8801]
TTGGTAACAATTGGCGATAGAGTCCCCGAACGGGGTGATATTATCCGATTAGAATTGAATCCCCCCACAGGAAGCGAACA
AGCGGGTTATCGTCCAGCTTTAATTATTTCCCCTGTCGCTTACAACCGCATCTCTAAACTGATTTTAATTTGTCCCATTA
CTAGCCGTCAAAAAGGTTGGCCATTTGAAGTTCTTTTACCCCCATCCTTGCAAACTTATGGGGTAGTTTTAGTCGATCAA
ATCCGTAGTATTGATTGTCAGGCAAGAAAGGCGGATTTTGTGGAAATTTTACCTGCTGATGTCATGGATGAAGTATTAGC
AAAATTAGAGCCTTTGTTAACCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9221.43 Da
• Isoelectric Point: 5.0631

>AT23288 WP_012594541.1 NC_011726:c1248325-1248083 [Rippkaea orientalis PCC 8801]
MTTQKISKWGNSLGIRLPHSMIQQLGWQEGVKVTLSTEGDRLILSSTKPKYYLDDLLKEITPEQQHEEIHWGEAVGEETW

Nucleotide

• Length: 243 bp

>AT23288 NC_011726:c1248325-1248083 [Rippkaea orientalis PCC 8801]
ATGACGACTCAAAAAATCAGCAAATGGGGAAATTCTCTGGGAATTAGACTGCCTCACAGCATGATTCAGCAATTAGGATG
GCAAGAAGGGGTAAAAGTAACCTTATCAACAGAAGGCGATCGCTTAATTCTGTCTTCCACCAAACCGAAATATTATCTTG
ATGACTTGCTAAAAGAGATAACTCCCGAACAGCAACACGAAGAAATCCATTGGGGAGAAGCAGTAGGAGAAGAAACTTGG
TAA

Structures

Toxin

• AlphaFold DB: B7K2D0

Antitoxin

• AlphaFold DB: B7K2D1