Detailed information of TA system TA232879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 34025..34515
• Replicon: plasmid pUK045_1
• Accession: NZ_CP091197
• Organism: Enterococcus faecalis strain UK045

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3GZA4
• Locus tag: L2629_RS00190
• Protein ID: WP_002358470.1
• Coordinates: 34246..34515 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L2629_RS00185
• Protein ID: WP_229240809.1
• Coordinates: 34025..34246 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2629_RS00170 (L2629_00165)	31698..32378	-	681	WP_236334723.1	IS6-like element IS1216 family transposase	-
L2629_RS00175 (L2629_00170)	32458..32721	+	264	WP_236334746.1	hypothetical protein	-
L2629_RS00180 (L2629_00175)	32724..33959	+	1236	WP_010717251.1	MobP2 family relaxase	-
L2629_RS00185 (L2629_00180)	34025..34246	+	222	WP_229240809.1	DUF6290 family protein	Antitoxin
L2629_RS00190 (L2629_00185)	34246..34515	+	270	WP_002358470.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L2629_RS00195 (L2629_00190)	34582..36162	+	1581	WP_002404807.1	DUF3991 domain-containing protein	-
L2629_RS00200 (L2629_00195)	36170..36883	+	714	WP_002358472.1	hypothetical protein	-
L2629_RS00205 (L2629_00200)	36884..37729	+	846	WP_002358473.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90994	90994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-69)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10610.17 Da
• Isoelectric Point: 10.5395

>T232879 WP_002358470.1 NZ_CP091197:34246-34515 [Enterococcus faecalis]
MSYRLEFTPDVKKQLKKMDKYQATLLTRWLYRNIDGTANPREHGKALSANRAGQWRYRIGNYRVIVEIEDERLVVTAIQV
GHRRNVYDK

Nucleotide

• Length: 270 bp

>T232879 NZ_CP128416:c4566657-4566376 [Rhizobium sp. CSC1952]
ATGAGCTATGAGCTCGCCTTTCTCGATGCCGCTTTGAAGGAATGGCGGAAGCTCGACCCCAATATCCGCGACCAGTTCAG
GAAGAAGCTTGCCGAGCGTCTTGAAAATCCGCGCGTACCCTCCGCACAGCTTTACGGGGCCAAGGACCGCTACAAGATCA
AGTTGAGGACCGCCGGTTACCGTCTCGTCTACGAGGTTCGCGATGCTCAGTTGATCGTGCTGGTGATTGCGGTTGGCCGC
AGGGACAGGAACGCAGTCTACAAGGCCGCGGGGAAGCGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8583.63 Da
• Isoelectric Point: 4.0179

>AT232879 WP_229240809.1 NZ_CP091197:34025-34246 [Enterococcus faecalis]
MITVRVSDEEKEWLSYMAEFFGVTLSDLLKSYSMEQLEDEYDKQTAEIAHKRWIEQGKETISMDEILNEFGGL

Nucleotide

• Length: 222 bp

>AT232879 NZ_CP128416:c4566895-4566647 [Rhizobium sp. CSC1952]
ATGGTCAGCGTCGTACTTGCGGAAGTTACCGCTAGCGTATCCGAACTGAAGCGCAACCCGATGGGAACCGTTGCCGCGGG
TGACGGAGCCCCCGTCGCCATTCTCAACCGCAATGAGCCGGCCTTCTATTGCATTCCGGCGAAGGCCTACGAAGTCATGA
TGGACCGGCTGGAGGATATGGAACTCAATGCGCTGGCCGATAGCCGGGCAGGGGAAGCCATCGTCAAGGTGACGCTGGAT
GAGCTATGA

Structures

Toxin

• AlphaFold DB: R3GZA4