Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 26527..27137 Replicon plasmid pVA_O1_2
Accession NZ_CP091188
Organism Vibrio anguillarum strain J382

Toxin (Protein)


Gene name PumA Uniprot ID A0A3M7LLW0
Locus tag L2O48_RS19510 Protein ID WP_038529385.1
Coordinates 26527..26823 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID Q6W4R6
Locus tag L2O48_RS19515 Protein ID WP_011154656.1
Coordinates 26847..27137 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2O48_RS19495 (L2O48_19495) 21784..22698 - 915 WP_011154651.1 helicase RepA family protein -
L2O48_RS19500 (L2O48_19500) 22695..23720 - 1026 WP_020977927.1 hypothetical protein -
L2O48_RS19505 (L2O48_19505) 24640..25888 - 1249 Protein_21 IS91-like element ISVa3 family transposase -
L2O48_RS19510 (L2O48_19510) 26527..26823 + 297 WP_038529385.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L2O48_RS19515 (L2O48_19515) 26847..27137 + 291 WP_011154656.1 putative addiction module antidote protein Antitoxin
L2O48_RS19520 (L2O48_19520) 27268..28482 - 1215 WP_020977929.1 hypothetical protein -
L2O48_RS19525 (L2O48_19525) 28484..28867 - 384 WP_011154658.1 hypothetical protein -
L2O48_RS19530 (L2O48_19530) 28873..29844 - 972 WP_011154659.1 ParB/RepB/Spo0J family partition protein -
L2O48_RS19535 (L2O48_19535) 29841..30581 - 741 WP_236531869.1 ParA family protein -
L2O48_RS19780 30864..31628 + 765 WP_268968793.1 DGQHR domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - bauB / bauB 1..76959 76959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(52-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11097.89 Da        Isoelectric Point: 10.7080

>T232878 WP_038529385.1 NZ_CP091188:26527-26823 [Vibrio anguillarum]
MFTIKRTQLFDDWLKTLKDAQARGAITARVQRLTQGLSGDVKPVGSGISELRIHVGKGYRVYFKRSGKQIVVLLCGGNKK
TQNQDIERAKELVQYFDL

Download         Length: 297 bp

>T232878 NZ_CP128416:4434752-4435045 [Rhizobium sp. CSC1952]
GTGCCACAAGTAATCGTCACCCGTGGCGCGATACTCGGGATCGAACGCTGCCGGACTTTCCTGAAGAAGGCCGGCGTGCA
GGTCTCTCGCCGCGCTTCGGACGCGATAAGCCGACAGTTTGCACTTTTGGAAACGTCGCCGGAAATCGGCCGGCCCTTTC
AGCATAATGCCGAACTGCGTGAACTGCTCATCCCCTTCGGGGCGTCCGGCTATATCGCCCTCTATCGCTATGAACCTGAC
GGTGACGCCGTCTATGTTCTGGCCTTCCGCCATCAGAGGGAAGTCCGCTATTAG

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10273.59 Da        Isoelectric Point: 4.3148

>AT232878 WP_011154656.1 NZ_CP091188:26847-27137 [Vibrio anguillarum]
MNITRLDDFDPADYLDNAESIQAYIDEAVATGDAAFISQSLGVIARAKGMNTLAEETGLSRETLYRTLSVKGNPNLKTLI
SITNALGVSVKLGSYC

Download         Length: 291 bp

>AT232878 NZ_CP128416:4434492-4434764 [Rhizobium sp. CSC1952]
ATGGCGACGTCACTCAAAATCGACGACGATCTCGAAGGCCGGGTCCAGAAGCTCGCCGTCTCGCGGCAGCAATCCACGCA
GGGCCTCATGCGTGAAGCCATCGAACAATATGTCGAACGAGAGGAAGCCCGCGAGAACTTCAAGCAGGAGGCGCTCGCTT
CCTGGGAAGACTACAAGGAGACCGGGCTCCATCTCACCGGAGATGAGCTTCGCGCCTGGATGAAGACATGGGGCACCGAG
GCTGAAACGGAAGCGCCTGAGTGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M7LLW0

Antitoxin

Source ID Structure
AlphaFold DB A0A3M7LK76

References