Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 41787..42397 Replicon plasmid pVA_O1_1
Accession NZ_CP091187
Organism Vibrio anguillarum strain J382

Toxin (Protein)


Gene name PumA Uniprot ID A0A3M7LLW0
Locus tag L2O48_RS19300 Protein ID WP_038529385.1
Coordinates 42101..42397 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID Q6W4R6
Locus tag L2O48_RS19295 Protein ID WP_011154656.1
Coordinates 41787..42077 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2O48_RS19275 (L2O48_19275) 38291..39085 + 795 WP_020977930.1 ParA family protein -
L2O48_RS19280 (L2O48_19280) 39082..40053 + 972 WP_011154659.1 ParB/RepB/Spo0J family partition protein -
L2O48_RS19285 (L2O48_19285) 40059..40442 + 384 WP_011154658.1 hypothetical protein -
L2O48_RS19290 (L2O48_19290) 40444..41658 + 1215 WP_020977929.1 hypothetical protein -
L2O48_RS19295 (L2O48_19295) 41787..42077 - 291 WP_011154656.1 putative addiction module antidote protein Antitoxin
L2O48_RS19300 (L2O48_19300) 42101..42397 - 297 WP_038529385.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L2O48_RS19305 (L2O48_19305) 42538..42849 - 312 WP_020977928.1 hypothetical protein -
L2O48_RS19310 (L2O48_19310) 43035..44282 + 1248 WP_013856937.1 IS91-like element ISVa3 family transposase -
L2O48_RS19315 (L2O48_19315) 45202..46227 + 1026 WP_020977927.1 hypothetical protein -
L2O48_RS19320 (L2O48_19320) 46224..47138 + 915 WP_011154651.1 helicase RepA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - bauB 1..65573 65573


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(52-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11097.89 Da        Isoelectric Point: 10.7080

>T232877 WP_038529385.1 NZ_CP091187:c42397-42101 [Vibrio anguillarum]
MFTIKRTQLFDDWLKTLKDAQARGAITARVQRLTQGLSGDVKPVGSGISELRIHVGKGYRVYFKRSGKQIVVLLCGGNKK
TQNQDIERAKELVQYFDL

Download         Length: 297 bp

>T232877 NZ_CP128416:c3569705-3569388 [Rhizobium sp. CSC1952]
ATGTTCGAAATCTACTCGACAAAAATCTTTAGAACCTGGGTCGATGGTTTGAGAGACAGGCGCGCTGTCACTCGTATTTT
CGCCCGCATAGCCCGAGCGGAAGAAGGTAATCTTGGTGACGTAAAACCGGTTGGCGAGGGCGTCAGCGAGATGCGCATCC
ACTATGGACCTGGGTACAGAGTTTACTTTACTCGGCGGGGCAAGGTCGTTTTGCTTCTACTCTGTGGAGGCGACAAGGAC
AGCCAGGCCAGCGATATCGCCGAGGCAAAACGAGTGGCCCAGAATTCGGGAGAAATAAGCGAATGGCCTTGGAAATAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10273.59 Da        Isoelectric Point: 4.3148

>AT232877 WP_011154656.1 NZ_CP091187:c42077-41787 [Vibrio anguillarum]
MNITRLDDFDPADYLDNAESIQAYIDEAVATGDAAFISQSLGVIARAKGMNTLAEETGLSRETLYRTLSVKGNPNLKTLI
SITNALGVSVKLGSYC

Download         Length: 291 bp

>AT232877 NZ_CP128416:c3569403-3569101 [Rhizobium sp. CSC1952]
ATGGCCTTGGAAATAAGACGGTTCGATATCGCGGAGCATCTCAACTCCGACGAAGATATTCAGGAATTCATGGTCGCCGC
CTTCGAAGACGGCGACCCCGCCTTCATCGCCAAATGCCTCGGCATCGTCGCAAGAGCCCAGAACATGAGCAAGCTGGCGC
GCGATGTCGGCATGAGCCGTGCGGCGCTCTACAAGGCGCTGAGCGGCGAAGGAAATCCGGAATTCGCGACGATCTTGAAG
GTGATGAAGGCGCTCGGGCTCGAACTGACGCCGGTCCTCGCCAGGACGGAGGATGCCGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M7LLW0

Antitoxin

Source ID Structure
AlphaFold DB A0A3M7LK76

References