Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 220645..221411 Replicon chromosome
Accession NZ_CP091186
Organism Vibrio anguillarum strain J382

Toxin (Protein)


Gene name - Uniprot ID F7YRS3
Locus tag L2O48_RS15565 Protein ID WP_013867769.1
Coordinates 220645..221142 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID F7YRS4
Locus tag L2O48_RS15570 Protein ID WP_013867770.1
Coordinates 221139..221411 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2O48_RS15525 (L2O48_15525) 215687..215893 + 207 WP_013867765.1 DUF1289 domain-containing protein -
L2O48_RS15530 (L2O48_15530) 216013..216243 - 231 WP_013867766.1 PAS factor family protein -
L2O48_RS15535 (L2O48_15535) 216537..216773 - 237 WP_013867767.1 hypothetical protein -
L2O48_RS15540 (L2O48_15540) 217058..217546 + 489 WP_013867768.1 hypothetical protein -
L2O48_RS15545 (L2O48_15545) 217546..217668 + 123 Protein_219 DUF3265 domain-containing protein -
L2O48_RS15550 (L2O48_15550) 217951..218268 + 318 WP_011154648.1 hypothetical protein -
L2O48_RS15555 (L2O48_15555) 218265..218618 + 354 WP_011154649.1 IS66 family insertion sequence element accessory protein TnpB -
L2O48_RS15560 (L2O48_15560) 218678..220219 + 1542 WP_011154650.1 IS66-like element ISVa5 family transposase -
L2O48_RS15565 (L2O48_15565) 220645..221142 - 498 WP_013867769.1 GNAT family N-acetyltransferase Toxin
L2O48_RS15570 (L2O48_15570) 221139..221411 - 273 WP_013867770.1 DUF1778 domain-containing protein Antitoxin
L2O48_RS15575 (L2O48_15575) 222162..222941 + 780 WP_013867772.1 hypothetical protein -
L2O48_RS15580 (L2O48_15580) 222938..223858 - 921 WP_011154643.1 IS5-like element ISVa2 family transposase -
L2O48_RS15585 (L2O48_15585) 224499..224912 + 414 WP_228209534.1 DUF1090 domain-containing protein -
L2O48_RS15590 (L2O48_15590) 224916..225005 + 90 WP_019280977.1 DUF3265 domain-containing protein -
L2O48_RS15595 (L2O48_15595) 225033..225710 - 678 Protein_229 IS3 family transposase -
L2O48_RS15600 (L2O48_15600) 225895..226206 - 312 WP_013867786.1 stress response translation initiation inhibitor YciH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 187798..226585 38787
- inside IScluster/Tn - - 213124..225785 12661
- inside Integron - - 195137..224912 29775


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18525.25 Da        Isoelectric Point: 9.2555

>T232876 WP_013867769.1 NZ_CP091186:c221142-220645 [Vibrio anguillarum]
MMNTVLLDKVKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDNNSSHIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKYFYERYGFQAFQDAENKLFITIADV
RASLG

Download         Length: 498 bp

>T232876 NZ_CP128416:c3569403-3569101 [Rhizobium sp. CSC1952]
ATGGCCTTGGAAATAAGACGGTTCGATATCGCGGAGCATCTCAACTCCGACGAAGATATTCAGGAATTCATGGTCGCCGC
CTTCGAAGACGGCGACCCCGCCTTCATCGCCAAATGCCTCGGCATCGTCGCAAGAGCCCAGAACATGAGCAAGCTGGCGC
GCGATGTCGGCATGAGCCGTGCGGCGCTCTACAAGGCGCTGAGCGGCGAAGGAAATCCGGAATTCGCGACGATCTTGAAG
GTGATGAAGGCGCTCGGGCTCGAACTGACGCCGGTCCTCGCCAGGACGGAGGATGCCGCCTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9731.18 Da        Isoelectric Point: 8.6154

>AT232876 WP_013867770.1 NZ_CP091186:c221411-221139 [Vibrio anguillarum]
MATTLPRITARVDVDTQDLLTKAAAIAGMSSINSFVLSAAIEKAKQVIEREQALKLSQADAILLMDALDRPTAQNSKLRA
ASERYESKAQ

Download         Length: 273 bp

>AT232876 NZ_CP128416:c3569705-3569388 [Rhizobium sp. CSC1952]
ATGTTCGAAATCTACTCGACAAAAATCTTTAGAACCTGGGTCGATGGTTTGAGAGACAGGCGCGCTGTCACTCGTATTTT
CGCCCGCATAGCCCGAGCGGAAGAAGGTAATCTTGGTGACGTAAAACCGGTTGGCGAGGGCGTCAGCGAGATGCGCATCC
ACTATGGACCTGGGTACAGAGTTTACTTTACTCGGCGGGGCAAGGTCGTTTTGCTTCTACTCTGTGGAGGCGACAAGGAC
AGCCAGGCCAGCGATATCGCCGAGGCAAAACGAGTGGCCCAGAATTCGGGAGAAATAAGCGAATGGCCTTGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Y3QZT4

Antitoxin

Source ID Structure
AlphaFold DB A0A7X8U164

References