Detailed information of TA system TA232875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 160890..161851
• Replicon: chromosome
• Accession: NZ_CP091186
• Organism: Vibrio anguillarum strain J382

Toxin (Protein)

• Gene name: -
• Uniprot ID: F7YRB7
• Locus tag: L2O48_RS15190
• Protein ID: WP_013868567.1
• Coordinates: 161318..161851 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L2O48_RS15185
• Protein ID: WP_013868568.1
• Coordinates: 160890..161321 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2O48_RS15160 (L2O48_15160)	156306..157745	-	1440	WP_232473010.1	DEAD/DEAH box helicase family protein	-
L2O48_RS15165 (L2O48_15165)	158287..158601	+	315	WP_006073510.1	hypothetical protein	-
L2O48_RS15170 (L2O48_15170)	158643..159611	-	969	WP_076611895.1	IS30-like element ISVa6 family transposase	-
L2O48_RS15175 (L2O48_15175)	159726..159818	+	93	WP_072600976.1	DUF3265 domain-containing protein	-
L2O48_RS15180 (L2O48_15180)	159856..160371	+	516	WP_013868571.1	GNAT family N-acetyltransferase	-
L2O48_RS15185 (L2O48_15185)	160890..161321	+	432	WP_013868568.1	DUF1778 domain-containing protein	Antitoxin
L2O48_RS15190 (L2O48_15190)	161318..161851	+	534	WP_013868567.1	GNAT family N-acetyltransferase	Toxin
L2O48_RS15195 (L2O48_15195)	161861..161971	+	111	WP_088718077.1	DUF3265 domain-containing protein	-
L2O48_RS15200 (L2O48_15200)	162537..162818	+	282	WP_029190024.1	hypothetical protein	-
L2O48_RS15205 (L2O48_15205)	163326..163451	+	126	WP_013868562.1	DUF3265 domain-containing protein	-
L2O48_RS15210 (L2O48_15210)	163484..164053	+	570	WP_013868561.1	hypothetical protein	-
L2O48_RS15215 (L2O48_15215)	164204..164548	+	345	WP_013868560.1	hypothetical protein	-
L2O48_RS15220 (L2O48_15220)	164545..164661	+	117	WP_080749045.1	DUF3265 domain-containing protein	-
L2O48_RS15225 (L2O48_15225)	164701..165357	+	657	WP_013868559.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	158643..188676	30033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (59-135)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20222.47 Da
• Isoelectric Point: 9.1188

>T232875 WP_013868567.1 NZ_CP091186:161318-161851 [Vibrio anguillarum]
VSWGREFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSGQPLLNQKFAICAFYSVAPSSISRETLPT
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMYLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T232875 NZ_CP128416:c3466464-3466276 [Rhizobium sp. CSC1952]
ATGAAAAGTGCCGAGGTGATCGCGCTGCTGCGCAAGGACGGCTGGACGGAAGTGGGCCAGAAAGGTAGCCACGTTCAATT
CAAGCATCCTCACAAGCCAGGGCGCGTTACCGTTCCCCACCCGAAGCGCGATATTCCGCTCGGCACGCTGCGGAGTATAG
AGAAGCAAGCCGGGCTGAAATTGAGGTGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15859.40 Da
• Isoelectric Point: 7.0831

>AT232875 WP_013868568.1 NZ_CP091186:160890-161321 [Vibrio anguillarum]
VFTVCFLSSAVRCQPLSRALYSPSFRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLG
LKSLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 432 bp

>AT232875 NZ_CP128416:c3466273-3465866 [Rhizobium sp. CSC1952]
ATGAGCAACTATATCGGCCTTATCCATAAGGAAGCGGACAGCGATTACGGCGTGTCCTTTCCGGATTTCCCTGGCGCCGT
GACGGCAGGCTCGACGCTGGACGAGGCCCGTCGGATGGCCGAGGAGGCGCTCGCCTTTCATGTGGAAGGCATGATCGAGG
ATGGCGAGACCATTCCCGAGCCCTCAAGCCTCGAAAGCGTGATGGCAGACCCGGATAACCGTGACGCGGTAGCGATCCTT
GTCAGCCTGAAGACGCAAGCCGCAAAGGCCGTGCGCATCAACATCACGCTGCCGGAGGATATCCTGCAGCGGATCGACAG
GTTTGCGGAGGCGAACGGCCTCTCCCGCTCCGGTTTTCTGGCGCGTGCCGCGCGGCGCGAGATCGAGCGGAATACGACGC
CTAACTAA

Structures

Toxin

• AlphaFold DB: A0A241NLG3