Detailed information of TA system TA232872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 2374390..2375083
• Replicon: chromosome
• Accession: NZ_CP091176
• Organism: Aeromonas caviae strain SCLZS52

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: L1871_RS11375
• Protein ID: WP_000182276.1
• Coordinates: 2374390..2374746 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: L1871_RS11380
• Protein ID: WP_001172026.1
• Coordinates: 2374748..2375083 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1871_RS11335 (L1871_11330)	2369606..2370238	+	633	WP_000186237.1	type B-3 chloramphenicol O-acetyltransferase CatB3	-
L1871_RS11340 (L1871_11335)	2370323..2370775	+	453	WP_001749986.1	NAD(+)--rifampin ADP-ribosyltransferase Arr-3	-
L1871_RS11345 (L1871_11340)	2370998..2371345	+	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
L1871_RS11350 (L1871_11345)	2371339..2372178	+	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-
L1871_RS11355 (L1871_11350)	2372306..2372806	+	501	WP_000376623.1	GNAT family N-acetyltransferase	-
L1871_RS11360 (L1871_11355)	2373127..2373195	+	69	Protein_2175	hypothetical protein	-
L1871_RS11365 (L1871_11360)	2373313..2374077	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
L1871_RS11370 (L1871_11365)	2374217..2374288	-	72	Protein_2177	EAL domain-containing protein	-
L1871_RS11375 (L1871_11370)	2374390..2374746	+	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L1871_RS11380 (L1871_11375)	2374748..2375083	+	336	WP_001172026.1	helix-turn-helix transcriptional regulator	Antitoxin
L1871_RS11385 (L1871_11380)	2375098..2375433	+	336	WP_000741275.1	hypothetical protein	-
L1871_RS11390 (L1871_11385)	2375457..2375783	+	327	WP_000091614.1	hypothetical protein	-
L1871_RS11395 (L1871_11390)	2375780..2376160	+	381	WP_001054412.1	hypothetical protein	-
L1871_RS11400 (L1871_11395)	2376317..2376577	+	261	WP_239991406.1	DUF3391 domain-containing protein	-
L1871_RS23630	2376538..2376642	+	105	Protein_2184	hypothetical protein	-
L1871_RS11405 (L1871_11400)	2376838..2377530	+	693	WP_230679382.1	HD-GYP domain-containing protein	-
L1871_RS11410 (L1871_11405)	2377814..2378092	+	279	WP_010465808.1	hypothetical protein	-
L1871_RS11415 (L1871_11410)	2378254..2378814	-	561	WP_010674758.1	3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aph(6)-Id / aph(3'')-Ib / mph(A) / aac(3)-IId / blaTEM-1B / aac(6')-Ib-cr / blaOXA-1 / catB3 / sul1 / blaCTX-M-3 / tet(C)	flmH	2283561..2456680	173119

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T232872 WP_000182276.1 NZ_CP091176:2374390-2374746 [Aeromonas caviae]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T232872 NZ_CP128416:1389321-1389602 [Rhizobium sp. CSC1952]
ATGAAGCTTTTCTGGACGCGGCAAGCCCGTCTCGACCGTCGAGCAATTTTTGACTATATCCGCCGTGAAAACCCGATAGC
GGCTGTTGCCATGGATCATCAGTTCACGAAAGCAGCGGATCAATTGGCACGCCATCCACTATCCGGCCGCGCCGGCCGTG
TCACCGATACGCGCGAACTGCCCGCCCATCCCAGCTATTTACTGATCTATGACGTCAAGGAGGATGCGGTGCGCATCCTC
AATGTCATCCACACCGCGCGCCAATGGCCGCCGAGTGACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT232872 WP_001172026.1 NZ_CP091176:2374748-2375083 [Aeromonas caviae]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT232872 NZ_CP128416:1389043-1389324 [Rhizobium sp. CSC1952]
ATGGCTGAAGCGACATTCACGTTTCGCGTCGAGGAAGAGCTGAAGGCGGCATTCAGCGAAGCAGCGAAAGCACAGGATCA
GTCCGATGCGCAATTGCTACGTACCTTCATGCGTGACTATGTCCGGCAGCAACAGGAGACCGCAGAATACGATGACTGGT
TTCGCCGGAAGGTGCAGAAGGCAATCGATTCCGCCAATGCCGGTGACCTGATTTCGGGAGACGATGTCGAAGCCGAGTTC
GCTGCTTTACGCGAAGAGGCGCGAAAGTCTTCCGCTCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7