Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 64722..65269 Replicon plasmid p1NCPPB4222
Accession NZ_CP091148
Organism Pseudomonas avellanae strain NCPPB 4222

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag L2Y01_RS27835 Protein ID WP_003319696.1
Coordinates 64943..65269 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7V8M172
Locus tag L2Y01_RS27830 Protein ID WP_003319697.1
Coordinates 64722..64946 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y01_RS27785 (L2Y01_27785) 59722..60135 - 414 WP_249741670.1 TraR/DksA family transcriptional regulator -
L2Y01_RS27790 (L2Y01_27790) 60171..60395 - 225 WP_044424864.1 carbon storage regulator -
L2Y01_RS27795 (L2Y01_27795) 60451..60666 - 216 WP_024420033.1 hypothetical protein -
L2Y01_RS27800 (L2Y01_27800) 60709..61002 - 294 WP_147477515.1 hypothetical protein -
L2Y01_RS27805 (L2Y01_27805) 61114..61479 - 366 WP_152522141.1 PapB/FocB family fimbrial expression transcriptional regulator -
L2Y01_RS27810 (L2Y01_27810) 62054..62338 - 285 Protein_61 site-specific integrase -
L2Y01_RS27815 (L2Y01_27815) 62459..62782 - 324 WP_007253135.1 hypothetical protein -
L2Y01_RS27820 (L2Y01_27820) 62775..63473 - 699 WP_005734862.1 ParA family protein -
L2Y01_RS27825 (L2Y01_27825) 63817..64449 + 633 WP_024421175.1 recombinase family protein -
L2Y01_RS27830 (L2Y01_27830) 64722..64946 + 225 WP_003319697.1 antitoxin MazE family protein Antitoxin
L2Y01_RS27835 (L2Y01_27835) 64943..65269 + 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L2Y01_RS27840 (L2Y01_27840) 65341..66444 + 1104 WP_004663854.1 IS5-like element ISPsy19 family transposase -
L2Y01_RS27845 (L2Y01_27845) 66681..67482 + 802 Protein_68 IS3-like element IS51 family transposase -
L2Y01_RS27850 (L2Y01_27850) 67596..68183 - 588 WP_081591645.1 AvrPphF family type III effector -
L2Y01_RS27855 (L2Y01_27855) 68194..68589 - 396 WP_024420708.1 CesT family type III secretion system chaperone -
L2Y01_RS27860 (L2Y01_27860) 69398..69652 - 255 WP_249741671.1 hypothetical protein -
L2Y01_RS27865 (L2Y01_27865) 69673..69849 + 177 Protein_72 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..110191 110191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T232860 WP_003319696.1 NZ_CP091148:64943-65269 [Pseudomonas avellanae]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

>T232860 NZ_CP128389:2416131-2416283 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8471.61 Da        Isoelectric Point: 6.2008

>AT232860 WP_003319697.1 NZ_CP091148:64722-64946 [Pseudomonas avellanae]
MPTSHVNARVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp

>AT232860 NZ_CP128389:2415507-2416106 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAACAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8M172

References