Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/upstrm_HI1419-dnstrm_HI1420
Location 4514165..4514751 Replicon chromosome
Accession NZ_CP091147
Organism Pseudomonas avellanae strain NCPPB 4222

Toxin (Protein)


Gene name relE Uniprot ID A0A3M5U8A4
Locus tag L2Y01_RS20475 Protein ID WP_005616225.1
Coordinates 4514165..4514473 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S6TPV2
Locus tag L2Y01_RS20480 Protein ID WP_017702466.1
Coordinates 4514470..4514751 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y01_RS20455 (L2Y01_20455) 4509998..4510318 - 321 WP_003382549.1 hypothetical protein -
L2Y01_RS20460 (L2Y01_20460) 4510532..4511743 - 1212 WP_024420796.1 HAMP domain-containing sensor histidine kinase -
L2Y01_RS20465 (L2Y01_20465) 4511877..4513052 - 1176 WP_005616230.1 PAS domain-containing sensor histidine kinase -
L2Y01_RS20470 (L2Y01_20470) 4513042..4513863 - 822 WP_024420797.1 alpha/beta hydrolase -
L2Y01_RS20475 (L2Y01_20475) 4514165..4514473 + 309 WP_005616225.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L2Y01_RS20480 (L2Y01_20480) 4514470..4514751 + 282 WP_017702466.1 putative addiction module antidote protein Antitoxin
L2Y01_RS20485 (L2Y01_20485) 4514840..4515277 - 438 WP_005616222.1 nucleoside deaminase -
L2Y01_RS20490 (L2Y01_20490) 4515311..4515811 - 501 WP_024420798.1 hypothetical protein -
L2Y01_RS20495 (L2Y01_20495) 4515946..4516959 - 1014 WP_005616217.1 ABC transporter substrate-binding protein -
L2Y01_RS20500 (L2Y01_20500) 4517089..4517499 + 411 WP_005616213.1 GNAT family N-acetyltransferase -
L2Y01_RS20505 (L2Y01_20505) 4517524..4518063 - 540 WP_003382539.1 peptide deformylase -
L2Y01_RS20510 (L2Y01_20510) 4518110..4519081 - 972 WP_003382537.1 YihY/virulence factor BrkB family protein -
L2Y01_RS20515 (L2Y01_20515) 4519308..4519499 - 192 WP_005767547.1 CsbD family protein -
L2Y01_RS20520 (L2Y01_20520) 4519489..4519719 + 231 WP_020321520.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-89)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11586.39 Da        Isoelectric Point: 8.4866

>T232856 WP_005616225.1 NZ_CP091147:4514165-4514473 [Pseudomonas avellanae]
MNTLIRTHEFDAWLKGLKDVTARLRILNRIMAAQEGNFGDCEPVGHGISEMRIFVGPGYRVYYTRRAEVVYLLLCGGNKS
SQKRDIKLAKSILDEIEKGDVE

Download         Length: 309 bp

>T232856 NZ_CP128388:c2788361-2787900 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10424.95 Da        Isoelectric Point: 5.1299

>AT232856 WP_017702466.1 NZ_CP091147:4514470-4514751 [Pseudomonas avellanae]
MRELKVFEASDYLNNDEEIAEFLSISLEDPNPDVFLRALQEVAKARGMSQLARDAGINRESLYKALTPGAKPRYDTVLKV
MNALGMKLTAQHV

Download         Length: 282 bp

>AT232856 NZ_CP128388:c2788697-2788374 [Staphylococcus aureus]
ATGAGAACTTCAGCAGAAATAGGTAAATTAATAAAACAACTACGCAAAGAGAACAATATAAATTTAACTGATTTTGCAAC
TAAAATAGGTGTTAATAAATCTACATTATCAAGATATGAAAATGGTAGTAGAAAAATACCTATGGAAGATATAGCTGAAA
TCGCAAACGCATTGAATGTTACCCCAGAAAGTTTATTATTAAAAAATAAACAACCAGAAACTGAAATACAACATCGTGCA
GCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAA
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M5U8A4

Antitoxin

Source ID Structure
AlphaFold DB A0A3M4K267

References