Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4255944..4256555 Replicon chromosome
Accession NZ_CP091147
Organism Pseudomonas avellanae strain NCPPB 4222

Toxin (Protein)


Gene name PfiT Uniprot ID A0A3M5U4B5
Locus tag L2Y01_RS19225 Protein ID WP_005618755.1
Coordinates 4256205..4256555 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID I3W0K1
Locus tag L2Y01_RS19220 Protein ID WP_005618754.1
Coordinates 4255944..4256192 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y01_RS19195 (L2Y01_19195) 4251732..4252835 - 1104 WP_004663854.1 IS5-like element ISPsy19 family transposase -
L2Y01_RS19200 (L2Y01_19200) 4253021..4254001 - 981 WP_249740213.1 IS5 family transposase -
L2Y01_RS19205 (L2Y01_19205) 4254153..4254389 + 237 WP_029768505.1 hypothetical protein -
L2Y01_RS19210 (L2Y01_19210) 4254504..4254617 + 114 Protein_3793 carbon storage regulator -
L2Y01_RS19215 (L2Y01_19215) 4254650..4255612 - 963 Protein_3794 tyrosine-type recombinase/integrase -
L2Y01_RS19220 (L2Y01_19220) 4255944..4256192 + 249 WP_005618754.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
L2Y01_RS19225 (L2Y01_19225) 4256205..4256555 + 351 WP_005618755.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L2Y01_RS19230 (L2Y01_19230) 4257108..4257503 - 396 WP_268794352.1 SGNH hydrolase domain-containing protein -
L2Y01_RS19235 (L2Y01_19235) 4257607..4259217 - 1611 WP_249741600.1 acyltransferase family protein -
L2Y01_RS19240 (L2Y01_19240) 4259464..4260531 - 1068 WP_024419981.1 IS630-like element ISPsy32 family transposase -
L2Y01_RS19245 (L2Y01_19245) 4260778..4260930 + 153 Protein_3800 IS5/IS1182 family transposase -
L2Y01_RS19250 (L2Y01_19250) 4260937..4261128 + 192 Protein_3801 IS21 family transposase -
L2Y01_RS19255 (L2Y01_19255) 4261134..4261418 - 285 Protein_3802 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12907.72 Da        Isoelectric Point: 4.3039

>T232855 WP_005618755.1 NZ_CP091147:4256205-4256555 [Pseudomonas avellanae]
MNTFALRFTDVAQQSIEDQVEHLAITQGFSSAAQRIDTLIDAIQDKLLSTPLGHPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITDIAVLLVLGGKQSVEQALIRYCLLQPM

Download         Length: 351 bp

>T232855 NZ_CP128388:2421447-2421599 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9345.71 Da        Isoelectric Point: 9.8452

>AT232855 WP_005618754.1 NZ_CP091147:4255944-4256192 [Pseudomonas avellanae]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFA
HK

Download         Length: 249 bp

>AT232855 NZ_CP128388:2420823-2421422 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAACAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M5U4B5

Antitoxin

Source ID Structure
AlphaFold DB A0A3M3Z0L4

References