Detailed information of TA system TA232853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1914233..1914972
• Replicon: chromosome
• Accession: NZ_CP091147
• Organism: Pseudomonas avellanae strain NCPPB 4222

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L2Y01_RS08990
• Protein ID: WP_024420983.1
• Coordinates: 1914541..1914972 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4P6QNR9
• Locus tag: L2Y01_RS08985
• Protein ID: WP_007247668.1
• Coordinates: 1914233..1914544 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y01_RS08955 (L2Y01_08955)	1909377..1909817	-	441	WP_005616045.1	GNAT family N-acetyltransferase	-
L2Y01_RS08960 (L2Y01_08960)	1909920..1911263	-	1344	WP_004397566.1	30S ribosomal protein S12 methylthiotransferase RimO	-
L2Y01_RS08965 (L2Y01_08965)	1911550..1911816	+	267	WP_024421275.1	type II toxin-antitoxin system RelE/ParE family toxin	-
L2Y01_RS08970 (L2Y01_08970)	1911810..1912826	-	1017	WP_005614760.1	IS30-like element ISPsy38 family transposase	-
L2Y01_RS08975 (L2Y01_08975)	1912934..1913587	+	654	WP_024421027.1	type III effector HopAZ1	-
L2Y01_RS08980 (L2Y01_08980)	1913605..1913871	-	267	WP_235390788.1	hypothetical protein	-
L2Y01_RS08985 (L2Y01_08985)	1914233..1914544	+	312	WP_007247668.1	Arc family DNA-binding protein	Antitoxin
L2Y01_RS08990 (L2Y01_08990)	1914541..1914972	+	432	WP_024420983.1	type II toxin-antitoxin system VapC family toxin	Toxin
L2Y01_RS08995 (L2Y01_08995)	1915013..1915646	+	634	Protein_1780	recombinase family protein	-
L2Y01_RS09000 (L2Y01_09000)	1915921..1916790	+	870	WP_229687600.1	AvrD family protein	-
L2Y01_RS28475	1916783..1916962	+	180	WP_259645586.1	AvrD family protein	-
L2Y01_RS09005 (L2Y01_09005)	1917304..1917864	+	561	WP_249741648.1	histidine phosphatase family protein	-
L2Y01_RS09010 (L2Y01_09010)	1918093..1919160	+	1068	WP_032687125.1	IS630-like element ISPsy32 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1865097..1927441	62344
	-	flank	IS/Tn	-	-	1911810..1912826	1016
	-	flank	IS/Tn	-	-	1918429..1919160	731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

• Arc-like DNA binding domain (23-64)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15506.91 Da
• Isoelectric Point: 4.7010

>T232853 WP_024420983.1 NZ_CP091147:1914541-1914972 [Pseudomonas avellanae]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIAIAIALANGFTVATRDTGPFEAAGLNVINPWEA

Nucleotide

• Length: 432 bp

>T232853 NZ_CP128388:2240415-2240624 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11377.77 Da
• Isoelectric Point: 8.6240

>AT232853 WP_007247668.1 NZ_CP091147:1914233-1914544 [Pseudomonas avellanae]
MVSKVYSDTTDGKTGNYQVANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGL
TDKEHTLFENTRITSPARAVSFE

Nucleotide

• Length: 312 bp

>AT232853 NZ_CP128388:2240110-2240403 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A4P6QNR9