Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/HicB(antitoxin) |
| Location | 62263..62903 | Replicon | chromosome |
| Accession | NZ_CP091147 | ||
| Organism | Pseudomonas avellanae strain NCPPB 4222 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | A0A0P9RJE6 |
| Locus tag | L2Y01_RS00315 | Protein ID | WP_007253326.1 |
| Coordinates | 62263..62445 (+) | Length | 61 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | Q6J2J7 |
| Locus tag | L2Y01_RS00320 | Protein ID | WP_005622461.1 |
| Coordinates | 62478..62903 (+) | Length | 142 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L2Y01_RS00290 (L2Y01_00290) | 57474..58277 | - | 804 | WP_003417182.1 | IS21-like element ISPsy20 family helper ATPase IstB | - |
| L2Y01_RS00295 (L2Y01_00295) | 58270..59769 | - | 1500 | WP_103356851.1 | IS21-like element ISPsy20 family transposase | - |
| L2Y01_RS00300 (L2Y01_00300) | 59884..60363 | - | 480 | Protein_59 | type I restriction endonuclease subunit R | - |
| L2Y01_RS00305 (L2Y01_00305) | 60438..60812 | - | 375 | Protein_60 | transposase | - |
| L2Y01_RS00310 (L2Y01_00310) | 61132..61881 | + | 750 | WP_122212308.1 | IS5 family transposase | - |
| L2Y01_RS00315 (L2Y01_00315) | 62263..62445 | + | 183 | WP_007253326.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| L2Y01_RS00320 (L2Y01_00320) | 62478..62903 | + | 426 | WP_005622461.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| L2Y01_RS00325 (L2Y01_00325) | 63207..63374 | - | 168 | WP_169338176.1 | hypothetical protein | - |
| L2Y01_RS00330 (L2Y01_00330) | 63428..63790 | + | 363 | WP_024421005.1 | Rap1a/Tai family immunity protein | - |
| L2Y01_RS00335 (L2Y01_00335) | 64261..64551 | - | 291 | WP_081591740.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| L2Y01_RS00340 (L2Y01_00340) | 64514..64777 | - | 264 | WP_024421039.1 | ribbon-helix-helix protein, CopG family | - |
| L2Y01_RS00345 (L2Y01_00345) | 65585..66650 | - | 1066 | Protein_68 | IS630 family transposase | - |
| L2Y01_RS00350 (L2Y01_00350) | 67214..67477 | - | 264 | WP_010212495.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | IScluster/Tn | - | - | 57474..71771 | 14297 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 61 a.a. Molecular weight: 6822.93 Da Isoelectric Point: 10.5498
>T232851 WP_007253326.1 NZ_CP091147:62263-62445 [Pseudomonas avellanae]
MKCSEFRRWLQAQGAEFKAAKGSHFKVYLNGKATIFADHGSKEMHEGLRKSIIKQLGLKD
MKCSEFRRWLQAQGAEFKAAKGSHFKVYLNGKATIFADHGSKEMHEGLRKSIIKQLGLKD
Download Length: 183 bp
>T232851 NZ_CP128387:c2788347-2787886 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA
Antitoxin
Download Length: 142 a.a. Molecular weight: 15749.11 Da Isoelectric Point: 4.7523
>AT232851 WP_005622461.1 NZ_CP091147:62478-62903 [Pseudomonas avellanae]
MYQYPLELHQEPTGIWLSCPDIPEMNASGDTLDEALAEALNGMESAFSLYVDQRRKIPQASLPSGDELVMHLPALTVAKI
MLWNSMLDNGVSRAELARRLSCTRQVVDRLVDFLHTSKIEQVERALAVLGRRITLSIEQAA
MYQYPLELHQEPTGIWLSCPDIPEMNASGDTLDEALAEALNGMESAFSLYVDQRRKIPQASLPSGDELVMHLPALTVAKI
MLWNSMLDNGVSRAELARRLSCTRQVVDRLVDFLHTSKIEQVERALAVLGRRITLSIEQAA
Download Length: 426 bp
>AT232851 NZ_CP128387:c2788683-2788360 [Staphylococcus aureus]
ATGAGAACTTCAGCAGAAATAGGTAAATTAATAAAACAACTACGCAAAGAGAACAATATAAATTTAACTGATTTTGCAAC
TAAAATAGGTGTTAATAAATCTACATTATCAAGATATGAAAATGGTAGTAGAAAAATACCTATGGAAGATATAGCTGAAA
TCGCAAACGCATTGAATGTTACCCCAGAAAGTTTATTATTAAAAAATAAACAACCAGAAACTGAAATACAACATCGTGCA
GCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAA
GTAA
ATGAGAACTTCAGCAGAAATAGGTAAATTAATAAAACAACTACGCAAAGAGAACAATATAAATTTAACTGATTTTGCAAC
TAAAATAGGTGTTAATAAATCTACATTATCAAGATATGAAAATGGTAGTAGAAAAATACCTATGGAAGATATAGCTGAAA
TCGCAAACGCATTGAATGTTACCCCAGAAAGTTTATTATTAAAAAATAAACAACCAGAAACTGAAATACAACATCGTGCA
GCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAA
GTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0P9RJE6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A8B3GLX7 |