Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapB(antitoxin)
Location 66533..67107 Replicon plasmid p3NCPPB3491
Accession NZ_CP091146
Organism Pseudomonas avellanae strain NCPPB 3491

Toxin (Protein)


Gene name vapC Uniprot ID Q48BC5
Locus tag L2Y00_RS29815 Protein ID WP_003344395.1
Coordinates 66533..66910 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3M5T0X5
Locus tag L2Y00_RS29820 Protein ID WP_005622049.1
Coordinates 66907..67107 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y00_RS29795 (L2Y00_29795) 61549..63564 + 2016 WP_024421514.1 DotA/TraY family protein -
L2Y00_RS29800 (L2Y00_29800) 63633..64382 + 750 WP_122212308.1 IS5 family transposase -
L2Y00_RS29805 (L2Y00_29805) 64598..65248 + 651 WP_002556019.1 plasmid IncI1-type surface exclusion protein ExcA -
L2Y00_RS29810 (L2Y00_29810) 65341..66543 + 1203 WP_005622046.1 hypothetical protein -
L2Y00_RS29815 (L2Y00_29815) 66533..66910 - 378 WP_003344395.1 PIN domain-containing protein Toxin
L2Y00_RS29820 (L2Y00_29820) 66907..67107 - 201 WP_005622049.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L2Y00_RS29825 (L2Y00_29825) 67199..68284 + 1086 WP_024420283.1 thioredoxin fold domain-containing protein -
L2Y00_RS29830 (L2Y00_29830) 68271..70559 + 2289 WP_249740319.1 F-type conjugative transfer protein TrbC -
L2Y00_RS29835 (L2Y00_29835) 70637..71065 + 429 WP_024421070.1 GntR family transcriptional regulator -
L2Y00_RS29840 (L2Y00_29840) 71144..71362 + 219 WP_122349173.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..71760 71760
- flank IS/Tn - - 63633..64382 749


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-43)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13633.73 Da        Isoelectric Point: 6.4723

>T232850 WP_003344395.1 NZ_CP091146:c66910-66533 [Pseudomonas avellanae]
VKGVLVDTSVWVEHFRNNSPELVNLLSQDRVLIHPMVIGELACGTPPDRSNTLTDLGDLRGAQQPTVSEVIAFLNTHKLY
GLGCGLVDMTLLASALLSGTALWTLDKRLERLASRMAVSYQPPTH

Download         Length: 378 bp

>T232850 NZ_CP128387:2421436-2421588 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7459.65 Da        Isoelectric Point: 5.7809

>AT232850 WP_005622049.1 NZ_CP091146:c67107-66907 [Pseudomonas avellanae]
MRTTVTIDDALYQRALEVADPAMDKADLFREAVQTFVRIQAAKRLMALGATLPAMEDIDRRHEKAL

Download         Length: 201 bp

>AT232850 NZ_CP128387:2420812-2421411 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAACAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3GPE2

Antitoxin

Source ID Structure
AlphaFold DB A0A3M5T0X5

References