Detailed information of TA system TA023285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 50806..51322
• Replicon: plasmid pS22302
• Accession: NC_011668
• Organism: Shewanella baltica OS223

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B8EFR1
• Locus tag: SBAL223_RS22775
• Protein ID: WP_011979492.1
• Coordinates: 51038..51322 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B8EFR0
• Locus tag: SBAL223_RS22770
• Protein ID: WP_011979491.1
• Coordinates: 50806..51048 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBAL223_RS22735	46135..46311	+	177	WP_012592962.1	AlpA family phage regulatory protein	-
SBAL223_RS22740	46425..47054	-	630	WP_012592963.1	recombinase family protein	-
SBAL223_RS22745	47297..47566	+	270	WP_011979484.1	hypothetical protein	-
SBAL223_RS22750	47887..48225	-	339	WP_150102369.1	hypothetical protein	-
SBAL223_RS22755	48330..49004	-	675	WP_012592966.1	ParA family protein	-
SBAL223_RS22760	49441..50061	+	621	WP_012592968.1	DUF2913 family protein	-
SBAL223_RS22765	50154..50426	+	273	WP_012592969.1	hypothetical protein	-
SBAL223_RS22770	50806..51048	+	243	WP_011979491.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SBAL223_RS22775	51038..51322	+	285	WP_011979492.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SBAL223_RS22780	51610..54864	-	3255	WP_012592970.1	type I restriction endonuclease subunit R	-
SBAL223_RS22785	54861..56009	-	1149	WP_012592971.1	DUF4268 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..65448	65448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11079.11 Da
• Isoelectric Point: 10.5812

>T23285 WP_011979492.1 NC_011668:51038-51322 [Shewanella baltica OS223]
MIYKLVFDPRALKEWNKLGDPIRQQLKKKLAEILQHPHIGANKLRDLPDCYKIKLRTSGYRLVYQVQDERITVFVVAVGK
RERLAVYSTAEKRL

Nucleotide

• Length: 285 bp

>T23285 NC_011668:51038-51322 [Shewanella baltica OS223]
ATGATCTATAAGTTAGTATTTGATCCCCGCGCATTAAAGGAATGGAACAAGCTTGGAGATCCCATCAGGCAGCAGTTAAA
AAAGAAACTGGCCGAGATACTGCAACATCCTCATATCGGGGCTAACAAACTTAGAGATCTCCCTGACTGTTACAAAATCA
AGTTGCGTACATCAGGTTATCGCTTGGTATATCAAGTACAAGATGAACGCATTACCGTGTTTGTTGTGGCCGTAGGCAAG
CGTGAACGATTAGCGGTGTATTCAACAGCAGAAAAGCGCCTTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8816.24 Da
• Isoelectric Point: 4.4501

>AT23285 WP_011979491.1 NC_011668:50806-51048 [Shewanella baltica OS223]
MGTINIRIDDELKERSFAVLEKLGVSPSEALRQMLEYVATSGAMPFKPVLLTAEDEALISLVKERLASPRKGIEVSLDDL

Nucleotide

• Length: 243 bp

>AT23285 NC_011668:50806-51048 [Shewanella baltica OS223]
ATGGGCACTATTAATATTCGCATCGATGACGAGCTTAAGGAGCGTTCTTTTGCTGTGTTGGAAAAATTAGGCGTTAGTCC
CTCTGAGGCATTGCGGCAAATGCTTGAGTATGTCGCCACAAGCGGCGCTATGCCATTTAAACCCGTACTGCTAACAGCAG
AGGATGAAGCCTTGATCTCCTTGGTTAAAGAGCGTTTAGCGAGCCCTCGAAAGGGGATCGAGGTTTCGCTCGATGATCTA
TAA

Structures

Toxin

• AlphaFold DB: A9L6M1

Antitoxin

• AlphaFold DB: A9L6M0