Detailed information of TA system TA232849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 26669..27336
• Replicon: plasmid p3NCPPB3491
• Accession: NZ_CP091146
• Organism: Pseudomonas avellanae strain NCPPB 3491

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3M5U379
• Locus tag: L2Y00_RS29580
• Protein ID: WP_005622134.1
• Coordinates: 26669..27085 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3M5U3K4
• Locus tag: L2Y00_RS29585
• Protein ID: WP_024421297.1
• Coordinates: 27082..27336 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y00_RS29545 (L2Y00_29545)	21731..22486	+	756	WP_151318986.1	IS21-like element ISPsy14 family helper ATPase IstB	-
L2Y00_RS29550 (L2Y00_29550)	22666..22866	+	201	WP_004666908.1	hypothetical protein	-
L2Y00_RS29555 (L2Y00_29555)	22933..23211	-	279	WP_004661031.1	mobilization protein	-
L2Y00_RS29560 (L2Y00_29560)	23255..23536	-	282	WP_011106972.1	hypothetical protein	-
L2Y00_RS29565 (L2Y00_29565)	23509..23853	-	345	WP_005622131.1	hypothetical protein	-
L2Y00_RS29570 (L2Y00_29570)	24113..24442	+	330	WP_003344297.1	plasmid mobilization protein MobA	-
L2Y00_RS29575 (L2Y00_29575)	24453..26408	+	1956	WP_024421296.1	relaxase/mobilization nuclease domain-containing protein	-
L2Y00_RS29580 (L2Y00_29580)	26669..27085	-	417	WP_005622134.1	type II toxin-antitoxin system VapC family toxin	Toxin
L2Y00_RS29585 (L2Y00_29585)	27082..27336	-	255	WP_024421297.1	plasmid stabilization protein	Antitoxin
L2Y00_RS29590 (L2Y00_29590)	27908..28531	-	624	WP_005740414.1	glycoside hydrolase family 25 protein	-
L2Y00_RS29595 (L2Y00_29595)	29065..30246	-	1182	WP_024421298.1	glycoside hydrolase family 10 protein	-
L2Y00_RS29600 (L2Y00_29600)	30331..31626	-	1296	WP_024420278.1	glycoside hydrolase family 68 protein	-
L2Y00_RS29605 (L2Y00_29605)	31638..31823	-	186	WP_024420279.1	Com family DNA-binding transcriptional regulator	-
L2Y00_RS29610 (L2Y00_29610)	31752..32117	+	366	WP_249740317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71760	71760

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14754.98 Da
• Isoelectric Point: 4.8361

>T232849 WP_005622134.1 NZ_CP091146:c27085-26669 [Pseudomonas avellanae]
MIVLDTNVVSEAMKPESHLAVRAWLNDQAAETLYLSSVTLAELLFGIGALPAGKRKDMLAQALDGLMGLFRDRGLPFDID
AARRYADLAVTAKTCGRGFPTPDGYIAAIAASRGFIVASRDTAPYEAVGVSVINPWEV

Nucleotide

• Length: 417 bp

>T232849 NZ_CP128387:2259989-2260351 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9441.89 Da
• Isoelectric Point: 10.2880

>AT232849 WP_024421297.1 NZ_CP091146:c27336-27082 [Pseudomonas avellanae]
MAMLTVRNLPDDVHRALRVRAAQHGHSTEAEVREILAIAVKPETRVRLGEALAALGRKIGLTNEDFEVFNQVRDKTPAKP
LRFE

Nucleotide

• Length: 255 bp

>AT232849 NZ_CP128387:2259822-2259992 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A3M5U379

Antitoxin

• AlphaFold DB: A0A3M5U3K4