Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapB(antitoxin)
Location 80863..81437 Replicon plasmid p2NCPPB3491
Accession NZ_CP091145
Organism Pseudomonas avellanae strain NCPPB 3491

Toxin (Protein)


Gene name vapC Uniprot ID Q48BC5
Locus tag L2Y00_RS29330 Protein ID WP_003344395.1
Coordinates 80863..81240 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3M5T0X5
Locus tag L2Y00_RS29335 Protein ID WP_005622049.1
Coordinates 81237..81437 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y00_RS29300 (L2Y00_29300) 77089..77694 + 606 Protein_79 MFS transporter -
L2Y00_RS29305 (L2Y00_29305) 77795..78349 + 555 Protein_80 MFS transporter -
L2Y00_RS29310 (L2Y00_29310) 78520..78705 + 186 WP_003344336.1 hypothetical protein -
L2Y00_RS29315 (L2Y00_29315) 78727..79110 - 384 WP_003344338.1 helix-turn-helix transcriptional regulator -
L2Y00_RS29320 (L2Y00_29320) 79324..79857 + 534 WP_024420740.1 transcription termination/antitermination NusG family protein -
L2Y00_RS29325 (L2Y00_29325) 80099..80848 + 750 WP_122212308.1 IS5 family transposase -
L2Y00_RS29330 (L2Y00_29330) 80863..81240 - 378 WP_003344395.1 PIN domain-containing protein Toxin
L2Y00_RS29335 (L2Y00_29335) 81237..81437 - 201 WP_005622049.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L2Y00_RS29340 (L2Y00_29340) 81529..82614 + 1086 WP_024420283.1 thioredoxin fold domain-containing protein -
L2Y00_RS29345 (L2Y00_29345) 82601..84889 + 2289 WP_249740315.1 F-type conjugative transfer protein TrbC -
L2Y00_RS29350 (L2Y00_29350) 84917..85339 + 423 WP_024421149.1 hypothetical protein -
L2Y00_RS29355 (L2Y00_29355) 85360..85923 + 564 WP_005622240.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..87243 87243
- flank IS/Tn - - 80099..80848 749


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-43)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13633.73 Da        Isoelectric Point: 6.4723

>T232848 WP_003344395.1 NZ_CP091145:c81240-80863 [Pseudomonas avellanae]
VKGVLVDTSVWVEHFRNNSPELVNLLSQDRVLIHPMVIGELACGTPPDRSNTLTDLGDLRGAQQPTVSEVIAFLNTHKLY
GLGCGLVDMTLLASALLSGTALWTLDKRLERLASRMAVSYQPPTH

Download         Length: 378 bp

>T232848 NZ_CP128387:2240405-2240614 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7459.65 Da        Isoelectric Point: 5.7809

>AT232848 WP_005622049.1 NZ_CP091145:c81437-81237 [Pseudomonas avellanae]
MRTTVTIDDALYQRALEVADPAMDKADLFREAVQTFVRIQAAKRLMALGATLPAMEDIDRRHEKAL

Download         Length: 201 bp

>AT232848 NZ_CP128387:2240100-2240393 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3GPE2

Antitoxin

Source ID Structure
AlphaFold DB A0A3M5T0X5

References