Detailed information of TA system TA232847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 68894..69561
• Replicon: plasmid p2NCPPB3491
• Accession: NZ_CP091145
• Organism: Pseudomonas avellanae strain NCPPB 3491

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3M5U379
• Locus tag: L2Y00_RS29260
• Protein ID: WP_005622134.1
• Coordinates: 68894..69310 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3M5U3K4
• Locus tag: L2Y00_RS29265
• Protein ID: WP_024421297.1
• Coordinates: 69307..69561 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y00_RS29225 (L2Y00_29225)	63987..64823	+	837	WP_044423062.1	DUF932 domain-containing protein	-
L2Y00_RS29230 (L2Y00_29230)	64891..65091	+	201	WP_004666908.1	hypothetical protein	-
L2Y00_RS29235 (L2Y00_29235)	65158..65436	-	279	WP_004661031.1	mobilization protein	-
L2Y00_RS29240 (L2Y00_29240)	65480..65761	-	282	WP_011106972.1	hypothetical protein	-
L2Y00_RS29245 (L2Y00_29245)	65734..66078	-	345	WP_005622131.1	hypothetical protein	-
L2Y00_RS29250 (L2Y00_29250)	66338..66667	+	330	WP_003344297.1	plasmid mobilization protein MobA	-
L2Y00_RS29255 (L2Y00_29255)	66678..68633	+	1956	WP_024421296.1	relaxase/mobilization nuclease domain-containing protein	-
L2Y00_RS29260 (L2Y00_29260)	68894..69310	-	417	WP_005622134.1	type II toxin-antitoxin system VapC family toxin	Toxin
L2Y00_RS29265 (L2Y00_29265)	69307..69561	-	255	WP_024421297.1	plasmid stabilization protein	Antitoxin
L2Y00_RS29270 (L2Y00_29270)	70133..70756	-	624	WP_005740414.1	glycoside hydrolase family 25 protein	-
L2Y00_RS29275 (L2Y00_29275)	71290..72471	-	1182	WP_024421298.1	glycoside hydrolase family 10 protein	-
L2Y00_RS29280 (L2Y00_29280)	72556..73851	-	1296	WP_024420278.1	glycoside hydrolase family 68 protein	-
L2Y00_RS29285 (L2Y00_29285)	73863..74048	-	186	WP_024420279.1	Com family DNA-binding transcriptional regulator	-
L2Y00_RS29290 (L2Y00_29290)	73977..74342	+	366	WP_249740317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..87243	87243

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14754.98 Da
• Isoelectric Point: 4.8361

>T232847 WP_005622134.1 NZ_CP091145:c69310-68894 [Pseudomonas avellanae]
MIVLDTNVVSEAMKPESHLAVRAWLNDQAAETLYLSSVTLAELLFGIGALPAGKRKDMLAQALDGLMGLFRDRGLPFDID
AARRYADLAVTAKTCGRGFPTPDGYIAAIAASRGFIVASRDTAPYEAVGVSVINPWEV

Nucleotide

• Length: 417 bp

>T232847 NZ_CP128387:700174-700362 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9441.89 Da
• Isoelectric Point: 10.2880

>AT232847 WP_024421297.1 NZ_CP091145:c69561-69307 [Pseudomonas avellanae]
MAMLTVRNLPDDVHRALRVRAAQHGHSTEAEVREILAIAVKPETRVRLGEALAALGRKIGLTNEDFEVFNQVRDKTPAKP
LRFE

Nucleotide

• Length: 255 bp

>AT232847 NZ_CP128387:699886-700146 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A3M5U379

Antitoxin

• AlphaFold DB: A0A3M5U3K4