Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 71729..72276 Replicon plasmid p1NCPPB3491
Accession NZ_CP091144
Organism Pseudomonas avellanae strain NCPPB 3491

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag L2Y00_RS28695 Protein ID WP_003319696.1
Coordinates 71729..72055 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7V8M172
Locus tag L2Y00_RS28700 Protein ID WP_003319697.1
Coordinates 72052..72276 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y00_RS28665 (L2Y00_28665) 67149..67325 - 177 Protein_73 hypothetical protein -
L2Y00_RS28670 (L2Y00_28670) 67337..67600 + 264 WP_235654512.1 hypothetical protein -
L2Y00_RS28675 (L2Y00_28675) 68409..68804 + 396 WP_024420708.1 CesT family type III secretion system chaperone -
L2Y00_RS28680 (L2Y00_28680) 68815..69402 + 588 WP_081591645.1 AvrPphF family type III effector -
L2Y00_RS28685 (L2Y00_28685) 69516..70317 - 802 Protein_77 IS3-like element IS51 family transposase -
L2Y00_RS28690 (L2Y00_28690) 70554..71657 - 1104 Protein_78 IS5-like element ISPsy19 family transposase -
L2Y00_RS28695 (L2Y00_28695) 71729..72055 - 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L2Y00_RS28700 (L2Y00_28700) 72052..72276 - 225 WP_003319697.1 antitoxin MazE family protein Antitoxin
L2Y00_RS28705 (L2Y00_28705) 72549..73181 - 633 WP_024421175.1 recombinase family protein -
L2Y00_RS28710 (L2Y00_28710) 74082..74363 + 282 WP_019742102.1 DUF1778 domain-containing protein -
L2Y00_RS28715 (L2Y00_28715) 74360..74869 + 510 WP_019742103.1 GNAT family N-acetyltransferase -
L2Y00_RS28720 (L2Y00_28720) 74923..75153 - 231 WP_019742104.1 hypothetical protein -
L2Y00_RS28725 (L2Y00_28725) 75150..75779 - 630 WP_024421519.1 ParA family partition ATPase -
L2Y00_RS28730 (L2Y00_28730) 75927..76163 - 237 WP_019740456.1 helix-turn-helix transcriptional regulator -
L2Y00_RS28735 (L2Y00_28735) 76281..76928 + 648 WP_019740455.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..105919 105919


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T232846 WP_003319696.1 NZ_CP091144:c72055-71729 [Pseudomonas avellanae]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

>T232846 NZ_CP128262:51368-51520 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8471.61 Da        Isoelectric Point: 6.2008

>AT232846 WP_003319697.1 NZ_CP091144:c72276-72052 [Pseudomonas avellanae]
MPTSHVNARVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp

>AT232846 NZ_CP128262:c51318-51256 [Salmonella enterica subsp. enterica serovar Heidelberg]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8M172

References