Detailed information of TA system TA232844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 5916351..5916937
• Replicon: chromosome
• Accession: NZ_CP091143
• Organism: Pseudomonas avellanae strain NCPPB 3491

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A3M5U8A4
• Locus tag: L2Y00_RS26875
• Protein ID: WP_005616225.1
• Coordinates: 5916629..5916937 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6TPV2
• Locus tag: L2Y00_RS26870
• Protein ID: WP_017702466.1
• Coordinates: 5916351..5916632 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y00_RS26830 (L2Y00_26830)	5911383..5911613	-	231	WP_020321520.1	hypothetical protein	-
L2Y00_RS26835 (L2Y00_26835)	5911603..5911794	+	192	WP_005767547.1	CsbD family protein	-
L2Y00_RS26840 (L2Y00_26840)	5912021..5912992	+	972	WP_003382537.1	YihY/virulence factor BrkB family protein	-
L2Y00_RS26845 (L2Y00_26845)	5913039..5913578	+	540	WP_003382539.1	peptide deformylase	-
L2Y00_RS26850 (L2Y00_26850)	5913603..5914013	-	411	WP_005616213.1	GNAT family N-acetyltransferase	-
L2Y00_RS26855 (L2Y00_26855)	5914143..5915156	+	1014	WP_005616217.1	ABC transporter substrate-binding protein	-
L2Y00_RS26860 (L2Y00_26860)	5915291..5915791	+	501	WP_005616219.1	hypothetical protein	-
L2Y00_RS26865 (L2Y00_26865)	5915825..5916262	+	438	WP_005616222.1	nucleoside deaminase	-
L2Y00_RS26870 (L2Y00_26870)	5916351..5916632	-	282	WP_017702466.1	putative addiction module antidote protein	Antitoxin
L2Y00_RS26875 (L2Y00_26875)	5916629..5916937	-	309	WP_005616225.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L2Y00_RS26880 (L2Y00_26880)	5917239..5918060	+	822	WP_024420797.1	alpha/beta hydrolase	-
L2Y00_RS26885 (L2Y00_26885)	5918050..5919225	+	1176	WP_005616230.1	PAS domain-containing sensor histidine kinase	-
L2Y00_RS26890 (L2Y00_26890)	5919359..5920570	+	1212	WP_005616231.1	HAMP domain-containing sensor histidine kinase	-
L2Y00_RS26895 (L2Y00_26895)	5920784..5921104	+	321	WP_003382549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-89)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11586.39 Da
• Isoelectric Point: 8.4866

>T232844 WP_005616225.1 NZ_CP091143:c5916937-5916629 [Pseudomonas avellanae]
MNTLIRTHEFDAWLKGLKDVTARLRILNRIMAAQEGNFGDCEPVGHGISEMRIFVGPGYRVYYTRRAEVVYLLLCGGNKS
SQKRDIKLAKSILDEIEKGDVE

Nucleotide

• Length: 309 bp

>T232844 NZ_CP128262:2255-2533 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10424.95 Da
• Isoelectric Point: 5.1299

>AT232844 WP_017702466.1 NZ_CP091143:c5916632-5916351 [Pseudomonas avellanae]
MRELKVFEASDYLNNDEEIAEFLSISLEDPNPDVFLRALQEVAKARGMSQLARDAGINRESLYKALTPGAKPRYDTVLKV
MNALGMKLTAQHV

Nucleotide

• Length: 282 bp

>AT232844 NZ_CP128262:1986-2255 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A3M5U8A4

Antitoxin

• AlphaFold DB: A0A3M4K267