Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 4600689..4601329 Replicon chromosome
Accession NZ_CP091143
Organism Pseudomonas avellanae strain NCPPB 3491

Toxin (Protein)


Gene name hicA Uniprot ID A0A0P9RJE6
Locus tag L2Y00_RS20840 Protein ID WP_007253326.1
Coordinates 4600689..4600871 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q6J2J7
Locus tag L2Y00_RS20845 Protein ID WP_005622461.1
Coordinates 4600904..4601329 (+) Length 142 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y00_RS20815 (L2Y00_20815) 4595917..4597416 - 1500 WP_249740206.1 IS21-like element ISPsy20 family transposase -
L2Y00_RS20820 (L2Y00_20820) 4597532..4597930 - 399 Protein_4126 type I restriction endonuclease subunit R -
L2Y00_RS20825 (L2Y00_20825) 4598005..4598379 - 375 Protein_4127 transposase -
L2Y00_RS20830 (L2Y00_20830) 4598699..4599448 + 750 WP_122212308.1 IS5 family transposase -
L2Y00_RS20835 (L2Y00_20835) 4599558..4600307 + 750 WP_122212308.1 IS5 family transposase -
L2Y00_RS20840 (L2Y00_20840) 4600689..4600871 + 183 WP_007253326.1 type II toxin-antitoxin system HicA family toxin Toxin
L2Y00_RS20845 (L2Y00_20845) 4600904..4601329 + 426 WP_005622461.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
L2Y00_RS20850 (L2Y00_20850) 4601633..4601800 - 168 WP_169338176.1 hypothetical protein -
L2Y00_RS20860 (L2Y00_20860) 4603938..4604954 + 1017 WP_005615738.1 IS30-like element ISPsy38 family transposase -
L2Y00_RS20865 (L2Y00_20865) 4605030..4605320 - 291 WP_081591740.1 type II toxin-antitoxin system RelE/ParE family toxin -
L2Y00_RS20870 (L2Y00_20870) 4605283..4605546 - 264 WP_024421039.1 ribbon-helix-helix protein, CopG family -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4588486..4609327 20841
- inside IScluster/Tn - - 4589561..4609327 19766


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-58)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6822.93 Da        Isoelectric Point: 10.5498

>T232841 WP_007253326.1 NZ_CP091143:4600689-4600871 [Pseudomonas avellanae]
MKCSEFRRWLQAQGAEFKAAKGSHFKVYLNGKATIFADHGSKEMHEGLRKSIIKQLGLKD

Download         Length: 183 bp

>T232841 NZ_CP128261:c4147699-4147415 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAAGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 142 a.a.        Molecular weight: 15749.11 Da        Isoelectric Point: 4.7523

>AT232841 WP_005622461.1 NZ_CP091143:4600904-4601329 [Pseudomonas avellanae]
MYQYPLELHQEPTGIWLSCPDIPEMNASGDTLDEALAEALNGMESAFSLYVDQRRKIPQASLPSGDELVMHLPALTVAKI
MLWNSMLDNGVSRAELARRLSCTRQVVDRLVDFLHTSKIEQVERALAVLGRRITLSIEQAA

Download         Length: 426 bp

>AT232841 NZ_CP128261:c4147931-4147689 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P9RJE6

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3GLX7

References