Detailed information of TA system TA232841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 4600689..4601329
• Replicon: chromosome
• Accession: NZ_CP091143
• Organism: Pseudomonas avellanae strain NCPPB 3491

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0P9RJE6
• Locus tag: L2Y00_RS20840
• Protein ID: WP_007253326.1
• Coordinates: 4600689..4600871 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q6J2J7
• Locus tag: L2Y00_RS20845
• Protein ID: WP_005622461.1
• Coordinates: 4600904..4601329 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y00_RS20815 (L2Y00_20815)	4595917..4597416	-	1500	WP_249740206.1	IS21-like element ISPsy20 family transposase	-
L2Y00_RS20820 (L2Y00_20820)	4597532..4597930	-	399	Protein_4126	type I restriction endonuclease subunit R	-
L2Y00_RS20825 (L2Y00_20825)	4598005..4598379	-	375	Protein_4127	transposase	-
L2Y00_RS20830 (L2Y00_20830)	4598699..4599448	+	750	WP_122212308.1	IS5 family transposase	-
L2Y00_RS20835 (L2Y00_20835)	4599558..4600307	+	750	WP_122212308.1	IS5 family transposase	-
L2Y00_RS20840 (L2Y00_20840)	4600689..4600871	+	183	WP_007253326.1	type II toxin-antitoxin system HicA family toxin	Toxin
L2Y00_RS20845 (L2Y00_20845)	4600904..4601329	+	426	WP_005622461.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
L2Y00_RS20850 (L2Y00_20850)	4601633..4601800	-	168	WP_169338176.1	hypothetical protein	-
L2Y00_RS20860 (L2Y00_20860)	4603938..4604954	+	1017	WP_005615738.1	IS30-like element ISPsy38 family transposase	-
L2Y00_RS20865 (L2Y00_20865)	4605030..4605320	-	291	WP_081591740.1	type II toxin-antitoxin system RelE/ParE family toxin	-
L2Y00_RS20870 (L2Y00_20870)	4605283..4605546	-	264	WP_024421039.1	ribbon-helix-helix protein, CopG family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4588486..4609327	20841
	-	inside	IScluster/Tn	-	-	4589561..4609327	19766

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6822.93 Da
• Isoelectric Point: 10.5498

>T232841 WP_007253326.1 NZ_CP091143:4600689-4600871 [Pseudomonas avellanae]
MKCSEFRRWLQAQGAEFKAAKGSHFKVYLNGKATIFADHGSKEMHEGLRKSIIKQLGLKD

Nucleotide

• Length: 183 bp

>T232841 NZ_CP128261:c4147699-4147415 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAAGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15749.11 Da
• Isoelectric Point: 4.7523

>AT232841 WP_005622461.1 NZ_CP091143:4600904-4601329 [Pseudomonas avellanae]
MYQYPLELHQEPTGIWLSCPDIPEMNASGDTLDEALAEALNGMESAFSLYVDQRRKIPQASLPSGDELVMHLPALTVAKI
MLWNSMLDNGVSRAELARRLSCTRQVVDRLVDFLHTSKIEQVERALAVLGRRITLSIEQAA

Nucleotide

• Length: 426 bp

>AT232841 NZ_CP128261:c4147931-4147689 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0P9RJE6

Antitoxin

• AlphaFold DB: A0A8B3GLX7