Detailed information of TA system TA023284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3827011..3827630
• Replicon: chromosome
• Accession: NC_011666
• Organism: Methylocella silvestris BL2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B8ETM1
• Locus tag: MSIL_RS17685
• Protein ID: WP_012592442.1
• Coordinates: 3827011..3827403 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MSIL_RS17690
• Protein ID: WP_012592443.1
• Coordinates: 3827400..3827630 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSIL_RS17670	3822981..3824477	+	1497	WP_012592439.1	UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanine ligase	-
MSIL_RS17675	3824479..3825561	+	1083	WP_012592440.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
MSIL_RS17680	3825564..3826949	+	1386	WP_012592441.1	UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase	-
MSIL_RS17685	3827011..3827403	-	393	WP_012592442.1	type II toxin-antitoxin system VapC family toxin	Toxin
MSIL_RS17690	3827400..3827630	-	231	WP_012592443.1	hypothetical protein	Antitoxin
MSIL_RS17695	3827868..3829064	+	1197	WP_012592444.1	putative lipid II flippase FtsW	-
MSIL_RS17700	3829061..3830176	+	1116	WP_012592445.1	undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase	-
MSIL_RS17705	3830173..3831579	+	1407	WP_012592446.1	UDP-N-acetylmuramate--L-alanine ligase	-
MSIL_RS17710	3831620..3832567	+	948	WP_012592447.1	UDP-N-acetylmuramate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (3-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14313.54 Da
• Isoelectric Point: 6.8812

>T23284 WP_012592442.1 NC_011666:c3827403-3827011 [Methylocella silvestris BL2]
MIVVDASALLEALLRTPSAKTVEDRLFAAGQTLHAPHLLDVEVAQVIRRYALSGEIDRARGGLALADLADLPLRRYPHDF
LLPRIWDLRNNLTAYDAAYVALAEALDAPLLTRDRRLAAAAGHRARIELV

Nucleotide

• Length: 393 bp

>T23284 NC_011666:c3827403-3827011 [Methylocella silvestris BL2]
TTGATCGTCGTCGACGCTTCCGCGCTTCTTGAAGCCCTTCTACGCACGCCCTCGGCGAAAACGGTGGAAGATCGGCTGTT
CGCCGCTGGTCAAACGCTTCACGCGCCGCATCTGCTCGATGTCGAAGTCGCGCAAGTGATCCGGCGTTACGCGTTGAGCG
GCGAGATCGACAGAGCGCGCGGCGGCTTGGCCCTCGCCGACCTGGCGGACCTTCCTTTGCGGCGATATCCGCACGATTTT
CTTCTGCCGCGCATTTGGGATTTGCGGAACAATCTGACGGCTTATGACGCCGCCTATGTCGCGCTGGCCGAGGCGCTTGA
CGCTCCGCTGCTGACGCGTGACCGGCGTTTAGCCGCGGCCGCCGGCCATCGCGCGCGGATCGAACTGGTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8626.84 Da
• Isoelectric Point: 8.7715

>AT23284 WP_012592443.1 NC_011666:c3827630-3827400 [Methylocella silvestris BL2]
MGVMIQIRNVPEALHRELKSRAALAGMSLSDYLLSEIRQTAERPTIEELRARLRSRTETDPSVPPAEAVRAERDRN

Nucleotide

• Length: 231 bp

>AT23284 NC_011666:c3827630-3827400 [Methylocella silvestris BL2]
ATGGGAGTAATGATCCAGATCCGCAATGTCCCCGAGGCGCTGCATCGCGAGTTGAAGTCGCGCGCCGCTCTGGCCGGCAT
GTCATTGTCCGACTATCTGCTCAGCGAGATTCGTCAGACAGCGGAGCGGCCGACGATTGAAGAGTTGCGGGCGCGCTTGC
GCAGCCGCACGGAAACGGATCCTTCCGTGCCCCCAGCCGAAGCGGTTCGCGCCGAGCGCGACCGCAATTGA

Structures

Toxin

• AlphaFold DB: B8ETM1