Detailed information of TA system TA232829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 186826..187493
• Replicon: chromosome
• Accession: NZ_CP091138
• Organism: Microbacterium sp. KUDC0406

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L2X99_RS01020
• Protein ID: WP_236125428.1
• Coordinates: 186826..187254 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L2X99_RS01025
• Protein ID: WP_236125427.1
• Coordinates: 187251..187493 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2X99_RS01000 (L2X99_01000)	182043..183698	-	1656	WP_236125431.1	malate synthase A	-
L2X99_RS01005 (L2X99_01005)	183990..185360	+	1371	WP_236126783.1	helix-turn-helix domain-containing protein	-
L2X99_RS01010 (L2X99_01010)	185491..186060	-	570	WP_236125430.1	TetR-like C-terminal domain-containing protein	-
L2X99_RS01015 (L2X99_01015)	186132..186842	+	711	WP_236125429.1	MBL fold metallo-hydrolase	-
L2X99_RS01020 (L2X99_01020)	186826..187254	-	429	WP_236125428.1	PIN domain-containing protein	Toxin
L2X99_RS01025 (L2X99_01025)	187251..187493	-	243	WP_236125427.1	hypothetical protein	Antitoxin
L2X99_RS01030 (L2X99_01030)	187521..188945	-	1425	WP_236135516.1	PucR family transcriptional regulator	-
L2X99_RS01035 (L2X99_01035)	189131..190489	+	1359	WP_236135517.1	4-aminobutyrate--2-oxoglutarate transaminase	-
L2X99_RS01040 (L2X99_01040)	190570..191952	+	1383	WP_236125424.1	NAD(P)/FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-134)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15208.41 Da
• Isoelectric Point: 4.5715

>T232829 WP_236125428.1 NZ_CP091138:c187254-186826 [Microbacterium sp. KUDC0406]
MIVVDVNVLIAAFRQDHPHHDVALPWLSQALSGSDDIVVPDAVWTGFLRIVTLPGAFRVPSRLDEASAFIHDVVSAGAYL
TAAPLIDGATHLVALCIETQATGNLVPDAYIASVALGYDCPVATFDRDFRRFDGLRIITPAA

Nucleotide

• Length: 429 bp

>T232829 NZ_CP128260:5388290-5388544 [Pseudomonas fluorescens]
ATGGACACTATCAACTACACCACGGCTCGTGCGCACTTGGCTGAAACCATGGATCGCGTGAATGAAGACTGCGCCCCACT
TCTGGTAACCCGCCAAAAAGGCGAGCCAGTAGTGATGATGTCTCTGGCCGAATACAACGCGCTGGAAGAAACGGCTTATC
TGCTGCGCTCTCCGGCCAATGCCGAGCGCTTGATCAAATCGATTGGCGAAATGCGCGCTGGAAAAGCGCAAGTCAGGCAA
CTGATCGAAGAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8456.60 Da
• Isoelectric Point: 9.9677

>AT232829 WP_236125427.1 NZ_CP091138:c187493-187251 [Microbacterium sp. KUDC0406]
MRTTLSINDRLLAQAKARAQESGVTLGAYVEEVLRRDLAASRPARRPVTLTVSAATGGAQPGVDLTTNRGLYEAMGTEIA

Nucleotide

• Length: 243 bp

>AT232829 NZ_CP128260:5388541-5388795 [Pseudomonas fluorescens]
ATGAAAATCCAGTTCACGCCAACGGGCTGGGAAGACTACTTGTGGTTTCAACAAAACGATAAGGCCGGTCTCAAACGAAT
CAATCTTCTGATCAAAGCGATCCAGCGCCAACCCTTTGAAGGCTTGGGTAAACCGGAGCCGCTCAAGCACAACATGAGCG
GCTTCTGGTCACGGCGGATAACTGCCGAGCATCGCTTGGTCTATGCAATCGTAGACGGCGAAATCTGCGTCATAACTTGC
AGATTTCACTATTGA