232828

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 1656435..1657068 Replicon chromosome
Accession NZ_CP091131
Organism Weizmannia coagulans strain TM3

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag L1879_RS07925 Protein ID WP_013860680.1
Coordinates 1656718..1657068 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag L1879_RS07920 Protein ID WP_014096808.1
Coordinates 1656435..1656713 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1879_RS07900 1652532..1653131 - 600 WP_258920962.1 rhomboid family intramembrane serine protease -
L1879_RS07905 1653413..1653772 + 360 WP_258920963.1 holo-ACP synthase -
L1879_RS07910 1653905..1654921 + 1017 WP_258920964.1 outer membrane lipoprotein carrier protein LolA -
L1879_RS07915 1655131..1656282 + 1152 WP_258920965.1 alanine racemase -
L1879_RS07920 1656435..1656713 + 279 WP_014096808.1 hypothetical protein Antitoxin
L1879_RS07925 1656718..1657068 + 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L1879_RS07930 1657183..1657323 - 141 WP_003352379.1 ribbon-helix-helix protein, CopG family -
L1879_RS07935 1657411..1658526 + 1116 WP_258920966.1 transposase -
L1879_RS07940 1659210..1660037 + 828 WP_258920967.1 RsbT co-antagonist protein RsbRA -
L1879_RS07945 1660041..1660397 + 357 WP_017553290.1 STAS domain-containing protein -
L1879_RS07950 1660402..1660803 + 402 WP_013860676.1 anti-sigma regulatory factor -
L1879_RS07955 1660818..1661861 + 1044 WP_061574529.1 PP2C family protein-serine/threonine phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1657411..1658526 1115


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T232828 WP_013860680.1 NZ_CP091131:1656718-1657068 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T232828 NZ_CP128260:4247089-4247433 [Pseudomonas fluorescens]
ATGGATGCTCTTTTTATCGAATTACCCGCGTTCCAGAAGCACCGGGACGATTACCTGGATGATGATCTGTTTCGCAGCTT
TCAGTTGGAGCTGCTAAAAAATCCTGAAGCGGGCGACCTCATCGAAGGCACGGGCGGGCTGCGTAAAATTCGCTTCTGCG
ACCAACGACGCGGTAAGGGCAAGCGCAGCGGATTACGAGTGATTTATTACTGGTGGTCTGGTTTCGACCAATTCTGGCTG
TTCACCGTGTACAGCAAAAACGAGCAAGACGATCTGTTGCCTTCCCAGAAAAGAGTCTTCAAACAGGCGCTGGATACAGA
AATCAACACGAGAACCCAAATATGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT232828 WP_014096808.1 NZ_CP091131:1656435-1656713 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT232828 NZ_CP128260:4247430-4247744 [Pseudomonas fluorescens]
ATGAAACGTGACATTTTTTCCGAACTGATGGAGGGCCTTGAGGCTTTGGCCGACGAGCGCCAAAGCAAGGTCACCCTTCG
CACCCACAAAGTCCAGTTACCCAAACTATTGCCGATCACTGCCGAGGAAGTTGTTGCGATCCGCCAGCAACTCAACCTCT
CCCGGTCAGTTTTCGCCATGTACCTGCGAACCAACACTAGAACCCTCGAAAACTGGGAACAAGGTCGAGCAACGCCAAAT
GCCCAAGCGACGACACTCATTCGACTGGTCGAGCGTTTTCCACAAACGATCGACCACCTTGCGGCTCTGACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References